HPLC Analysis of Allantoin on SeQuant® ZIC-HILIC
CONDITIONS
column
SeQuant ZIC-HILIC, PEEK, 150 x 4.6 mm, 5 μm, 200 Å (1.50455.0001)
mobile phase
[A] 0.1% (v/v) formic acid; [B] acetonitrile; (25:75, A:B)
flow rate
0.5 mL/min
pressure
232 psi (16 bar)
column temp.
30 °C
detector
210 nm
injection
20 μL
sample
0.03 mg/ml in Mobile phase
Description
Legal Information
SeQuant is a registered trademark of Merck KGaA, Darmstadt, Germany