HPLC Analysis of Substance P and Its Fragments on Discovery^® C18

column

Discovery C18, 15 cm × 4.6 mm I.D., 5 μm particles (504955)

mobile phase

[A] water + 0.1% TFA; [B] acetonitrile + 0.1% TFA

gradient

linear; 5-29%B (6 min); 29-34B (5 min)

flow rate

1 mL/min

column temp.

35 °C

detector

UV, 220 nm

sample

Substance P in initial mobile phase