LC/MS Analysis of Fructose and NaCl on SeQuant^® ZIC-HILIC

column

SeQuant ZIC-cHILIC, PEEK, 150 x 4.6 mm, 5 μm (1.50461.0001)

mobile phase

[A] acetonitrile; [B] 100 mM Ammonium acetate, adjusted with acetic acid to pH 5.0; (80:20, A:B)

flow rate

1.5 mL/min

pressure

2000 psi 135 Bar

column temp.

40 °C

detector

ELSD (Analytical nebulizer)

injection

20 μL

sample

1000 ppm Fructose and 90 ppm NaCl (Diluent Acetonitrile and water 80:20 (v/v))