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LC/MS (TOF) Analysis of DL-α-Hydroxyglutaric Acid Enantiomers on Astec® CHIROBIOTIC® R, Fast Separation

LC/MS (TOF) Analysis of DL-α-Hydroxyglutaric Acid Enantiomers on Astec® CHIROBIOTIC® R, Fast Separation application for HPLC

Materials

Analytical column

Product No.
Description
Pricing

Astec® CHIROBIOTIC® R Chiral HPLC Column

5 μm particle size, L × I.D. 10 cm × 4.6 mm

Standard

Product No.
Description
Pricing

D-α-Hydroxyglutaric acid disodium salt

≥95% (GC)

L-α-Hydroxyglutaric acid disodium salt

≥98.0% (GC)

DL-α-Hydroxyglutaric acid disodium salt

≥95.0% (GC)

CONDITIONS

column

Astec CHIROBIOTIC® R, 10 cm x 4.6 mm I.D., 5μm particles (13022AST)

mobile phase

5:95, (water:methanol) with 0.3% acetic acid and 0.1% ammonium hydroxide

flow rate

1 mL/min

pressure

1700 psi (117 bar)

column temp.

25 °C

detector

MS, ESI(-) TOF, SIM, m/z 147.03, split 100:1

injection

10 μL

sample

DL-a-Hydroxyglutaric acid, 1 mg/mL in 50:50, water:methanol

Description

Analysis Note

Chiral 2-hydroxyglutarates (2-OHG) are important molecular signatures of both healthy and diseased biological cells, their specific biochemical pathways and inborn errors of metabolism. The chiral differentiation and quantification of D-2-OHG and L-2-OHG is key for characterizing neurometabolic disorders like the 2-hydroxyglutaric acidurias that cause neurological impairment early in life. In this application, an Astec® CHIROBIOTIC® R column run under polar ionic mobile phase conditions achieved a rapid separation of the D and L enantiomers of 2-OHG under conditions amenable for sensitive LC-MS/MS detection.

Legal Information

Astec is a registered trademark of Merck KGaA, Darmstadt, Germany
CHIROBIOTIC is a registered trademark of Sigma-Aldrich Co. LLC