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UHPLC/MS Analysis of Heroin and Related Compounds on Titan C18

UHPLC/MS Analysis of Heroin and Related Compounds on Titan™ C18 application for UHPLC

CONDITIONS

column

Titan C18 5 cm x 2.1 mm I.D., 1.9 μm particles (577122-U)

mobile phase

[A] 5 mM ammonium acetate in 95:5, water:acetonitrile; [B] 5 mM ammonium acetate in 5:95, water:acetonitrile

gradient

0 to 100% B in 3 min, held at 100% B for 1 min

flow rate

0.6 mL/min

pressure

6770 psi (467 bar)

column temp.

35 °C

detector

MS-TOF, XIC

injection

0.5 μL

sample

1 μg/mL in 95:5, water:methanol

Description

Analysis Note

This method shows heroin and related compounds separated on a Titan C18 column and MS detection. The highest quality LC-MS solvents were used to supply low background interference and low particulate contaminants for robust, trouble-free operation. Cerilliant CRMs provided reliable identification and quantification.

Legal Information

Titan is a trademark of Sigma-Aldrich Co. LLC