HomeDNA & RNA PurificationPlasmid DNA Preparation

Plasmid DNA Preparation

CSHL Press

Product lists for commonly used protocols from Cold Spring Harbor Protocols

Selected Protocol from "Molecular Cloning: A Laboratory Manual", Third Edition, Joseph Sambrook, David W. Russell.

Preparation of Plasmid DNA by Alkaline Lysis with SDS: Maxipreparation

Joseph Sambrook
Peter Maccallum Cancer Institute and The University of Melbourne, Australia
David W. Russell
University of Texas Southwestern Medical Center, Dallas


Plasmid DNA may be isolated from large-scale (500 mL) bacterial cultures by treatment with alkali and SDS. The resulting DNA preparation may be further purified by column chromatography or centrifugation through CsCl-ethidium bromide gradients.

Products Available for this Protocol

Plasmid DNA Preparation

Product Association Disclaimer: The products listed for this specific protocol were selected either to match or to supplement the products listed within the actual protocol. The products/reagents have been qualified for usage, but may not have been validated for this specific application. Please refer to the detailed product description on the usage of specific products of interest.

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