Glycobiology Analysis Manual, 2nd Edition

Lipopolysaccharide (LPS) is the major component of the outer membrane of Gram-negative bacteria. Lipopolysaccharide is localized in the outer layer of the membrane and is, in noncapsulated strains, exposed on the cell surface.


Intact bacterial lipopolysaccharides are macromolecules of molecular mass 10‑20 kDa made up of three structural components
(Figure 1):

  • A hydrophobic lipid section, lipid A, which is responsible for the toxic properties of the molecule,
  • A hydrophilic core polysaccharide chain, and
  • A repeating hydrophilic O-antigenic oligosaccharide side chain that is specific to the bacterial serotype.1
General structure for bacterial lipopolysaccharides

Figure 1.General structure for bacterial lipopolysaccharides. See the text for detailed explanation regarding each section. Abbreviations: KDO: 3‑deoxy-α-D-mannooctulosonic acid; Hep: Heptulose (ketoheptose); NGa: Galactosamine; NGc: Glucosamine.

The lipid A core is made up of a β-glucosamine-(1→6)-glucosamine-1-phosphate base with fatty acid esters attached to both carbohydrates. The acyl chain length and number of acyl groups may vary between bacterial species but are relatively conserved within a species. The inner polysaccharide core typically contains between 1 and 4 molecules of the KDO (3‑deoxy-α-D-manno-octulosonic acid) attached to the disaccharide core. KDO is specifically associated with lipopolysaccharide, and biologically active lipid A was thought to require at least one KDO residue for bacterial survival.2 However, an Escherichia coli K-12 suppressor strain that is KDO deficient demonstrates that the KDO requirement is not absolute for viability.3

The KDO-containing inner core also is modified with heptulose (ketoheptose) monosaccharides, the most common of which is L-glycero-α-D-manno-heptopyranose. The inner core glycan residues are typically phosphorylated or modified with phosphate-containing groups, e.g., pyrophosphate or 2‑aminoethylphosphate. The phosphate groups of lipopolysaccharides increase the overall negative charge of the cell membrane and help to stabilize the structure.

The outer core of the lipopolysaccharide contains more common hexoses, including glucose, galactose, and N-acetylglucosamine and is structurally more diverse than the inner core.

The O-antigen is a repeating oligosaccharide unit typically comprised of two to six sugars. The O-antigen is the primary structural constituent of lipopolysaccharide that differentiates bacteria. The distinctive O-antigen structures have been used to identify and assign serogroups to Escherichia coli, Salmonella enterica, and Vibrio cholerae.4 Lipopolysaccharides from rough mutant strains of E. coli lack the O-antigen portion of the structure.

The core section and the lipid A section of a lipopolysaccharide may have some variability in structure, while the O-antigen has a high degree of structural variability as well as variability in the number of repeating units. These differences result in a significant amount of heterogeneity in LPS preparations. Since LPS is heterogeneous and tends to form aggregates of varying sizes, there is a reported "molecular mass" range for these aggregates of 1‑4 million Dalton or greater. When the LPS is treated with sodium dodecyl sulfate (SDS) and heat, the molecular mass is ~50‑100 kDa.5

Functions and Applications

Within Gram-negative bacteria, the membrane lipopolysaccharides protect the bacterium against the action of bile salts and lipophilic antibiotics.6

Lipopolysaccharides are heat-stable endotoxins and have long been recognized as a key factor in septic shock (septicemia) in humans1,7 and, more generally, in inducing a strong immune response in normal mammalian cells. The lipid A moiety has been identified as critical to the endotoxin activity of lipopolysaccharide. This was demonstrated, Galanos, et al., by finding identical bioactive results, including endotoxic activity, between synthetic and natural-sourced E. coli lipid A preparations.8 The active receptor for lipopolysaccharide has been identified as the CD14/TLR4/MD2 receptor complex, which promotes the secretion of proinflammatory cytokines including tumor necrosis factor-α and interleukin-1.9 While the lipid A component is primarily responsible for immune response activation, the polysaccharide component of Salmonella enterica LPS is also necessary for NF-κB activation.10

Lipopolysaccharide preparations have been used in research for the elucidation of LPS structure,11 metabolism,12 immunology,13 physiology,14 toxicity,15 and biosynthesis.16 They have also been used to induce synthesis and secretion of growth-promoting factors such as interleukins.17,18 Because of its connection to septicemia, lipopolysaccharide has been studied to identify possible targets for antibodies and inhibitors to LPS biosynthesis.19,20

Extraction and Purification

Lipopolysaccharides can be prepared by extraction from TCA,21 phenol,22,23 or phenol-chloroform-petroleum ether (for rough strains).24 TCA extracted lipopolysaccharides are structurally similar to the phenol extracted ones, with similar electrophoretic patterns and endotoxicity. The primary differences are in the amounts of nucleic acid and protein contaminants remaining after extraction. The TCA extracts contain ~2% RNA and ~10% denatured proteins, while phenol extracts contain up to 60% RNA and <1% protein. Subsequent purification by gel filtration chromatography removes much of the protein present in the phenolextracted LPS but results in a preparation that contains 10‑20% nucleic acids. Further purification using ion exchange chromatography yields a lipopolysaccharide product that contains <1% protein and <1% RNA.



Rietschel ET, Kirikae T, Schade FU, Mamat U, Schmidt G, Loppnow H, Ulmer AJ, Zähringer U, Seydel U, Di Padova F, et al. 1994. Bacterial endotoxin: molecular relationships of structure to activity and function. FASEB j.. 8(2):217-225.
HELANDER IM, LINDNER B, BRADE H, ALTMANN K, LINDBERG AA, RIETSCHEL ET, ZAHRINGER U. 1988. Chemical structure of the lipopolysaccharide of Haemophilus influenzae strain I-69 Rd-/b+. Description of a novel deep-rough chemotype. Eur J Biochem. 177(3):483-492.
Meredith TC, Aggarwal P, Mamat U, Lindner B, Woodard RW. 2006. Redefining the Requisite Lipopolysaccharide Structure inEscherichia coli. ACS Chem. Biol.. 1(1):33-42.
Samuel G, Reeves P. 2003. Biosynthesis of O-antigens: genes and pathways involved in nucleotide sugar precursor synthesis and O-antigen assembly. Carbohydrate Research. 338(23):2503-2519.
JANN B, RESKE K, JANN K. 1975. Heterogeneity of Lipopolysaccharides. Analysis of Polysaccharide Chain Lengths by Sodium Dodecylsulfate-Polyacrylamide Gel Electrophoresis. Eur J Biochem. 60(1):239-246.
Mayer H, Tharanathan R, Weckesser J. 1985. 6 Analysis of Lipopolysaccharides of Gram-Negative Bacteria.157-207.
GALANOS C, LUDERITZ O, RIETSCHEL ET, WESTPHAL O, BRADE H, BRADE L, FREUDENBERG M, SCHADE U, IMOTO M, YOSHIMURA H, et al. 1985. Synthetic and natural Escherichia coli free lipid A express identical endotoxic activities. Eur J Biochem. 148(1):1-5.
Palsson-McDermott EM, O'Neill LAJ. 2004. Signal transduction by the lipopolysaccharide receptor, Toll-like receptor-4. Immunology. 113(2):153-162.
Muroi M, Tanamoto K. 2002. The Polysaccharide Portion Plays an Indispensable Role in Salmonella Lipopolysaccharide-Induced Activation of NF-?B through Human Toll-Like Receptor 4. IAI. 70(11):6043-6047.
Strain SM, Fesik SW, Armitage IM. 1983. Characterization of lipopolysaccharide from a heptoseless mutant of Escherichia coli by carbon 13 nuclear magnetic resonance.. Journal of Biological Chemistry. 258(5):2906-2910.
Munford RS, Andersen JM, Dietschy JM. 1981. Sites of tissue binding and uptake in vivo of bacterial lipopolysaccharide-high density lipoprotein complexes: studies in the rat and squirrel monkey.. J. Clin. Invest.. 68(6):1503-1513.
Morrison DC, Rudbach JA. 1981. Endotoxin-Cell-Membrane Interactions Leading to Transmembrane Signaling.187-218.
Galanos C. 1977. International Review of Biochemistry. Biochemistry of Lipids III Vol. 14, 2309. Baltimore, MD USA: University Park Press.
Kurtz HJ, Quast J. 1982. Effects of continuous intravenous infusion of Escherichia coli endotoxin into swine. 43(2). Am J Vet Res: 262-268.
Rick PD, Young DA. 1982. Isolation and characterization of a temperature-sensitive lethal mutant of Salmonella typhimurium that is conditionally defective in 3-deoxy-D-manno-octulosonate-8-phosphate synthesis.. 150(2):447-455.
Skelly RR, Munkenbeck P, Morrison DC. 1979. Stimulation of T-independent antibody responses by hapten-lipopolysaccharides without repeating polymeric structure.. 23(2):287-293.
Lang CH, Silvis C, Deshpande N, Nystrom G, Frost RA. 2003. Endotoxin Stimulates In Vivo Expression of Inflammatory Cytokines Tumor Necrosis Factor Alpha, Interleukin-1??, -6, and High-Mobility-Group Protein-1 in Skeletal Muscle. Shock. 19(6):538-546.
Müller-Loennies S, Brade L, MacKenzie C, Di Padova FE, Brade H. 2003. Identification of a Cross-reactive Epitope Widely Present in Lipopolysaccharide from Enterobacteria and Recognized by the Cross-protective Monoclonal Antibody WN1 222-5. Journal of Biological Chemistry. 278(28):25618-25627.

Staub A. Bacterial lipido-protinopolysaccharides ('O' somatic antigens) extraction with trichloroacetic acid in Methods in Carbohydrate Chem. Vol. 5, 92-93. Hoboken, NJ USA: John Wiley & Sons, Inc..
Westphal O, Jann K. 1965. Bacterial lipopolysaccharides extraction with phenolwater and further applications of the procedure in Methods in Carbohydrate Chem. Vol. 5, 92‑93. Hoboken, NJ USA: John Wiley & Sons, Inc.
Leive L, Morrison DC. 1972. [23] Isolation of lipopolysaccharides from bacteria.254-262.
Galanos C, Luderitz O, Westphal O. 1969. A New Method for the Extraction of R Lipopolysaccharides. Eur J Biochem. 9(2):245-249.