07-2173

Anti-Stat3 Antibody

from rabbit

• Synonym(s): Acute-phase response factor, DNA-binding protein APRF, signal transducer and activator of transcription 3, signal transducer and activator of transcription 3, (acute-phase response factor)
• UNSPSC Code: 12352203
• eCl@ss: 32160702

Properties

• biological source: rabbit
• Quality Level: 100
• conjugate: unconjugated
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: rat, pig, human, mouse
• species reactivity (predicted by homology): primate (based on 100% sequence homology), bovine (based on 100% sequence homology), porcine (based on 100% sequence homology)
• technique(s): immunoprecipitation (IP): suitable; western blot: suitable
• NCBI accession no.: NP_644805
• UniProt accession no.: P40763
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... STAT3(6774)

Description

General description

This protein is a member of the family of STAT proteins (Signal Transduction and Activators of Transcription). These proteins are latent cytoplasmic transcription factors with the dual function of signal transduction and activation of transcription. STATs are activated by tyrosine phosphorylation in response to different ligands, after which they translocate to the cell nucleus. The N-terminal region is highly homologous among the STAT proteins and surrounds a completely conserved arginine residue. The Stat3 protein is expressed in most cell types and is activated by multiple cytokines and growth factors including: IFN-a, IL-10, IL-6, IL-11, onM, LIF, CNTF, G-CSF, IL-12, IL-2, GH, EGF, PDGF, and CSF-1. Stat3 is not activated by IFN beta. One active Stat3 translocates to the nucleus and binds to DNA as either a homodimer or as a heterodimer with Stat1. Transcription factors associated with Stat3 include cJun and cyclic AMP responsive enhancer binding protein (CREB). Stat3 interacts with the APRE (acute phase response element) and the SIE (c-sis inducible element). These proteins are latent cytoplasmic transcription factors with the dual function of signal transduction and activation of transcription. STATs are activated by tyrosine phosphorylation in response to different ligands, after which they translocate to the cell nucleus. The N-terminal region is highly homologous among the STAT proteins and surrounds a completely conserved arginine residue.

~ 88 kDa observed.

Immunogen

Epitope: Near C-terminus

KLH-conjugated linear peptide corresponding to human STAT3 near the C-terminus.

Application

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Use Anti-Stat3 Antibody (rabbit polyclonal antibody) validated in WB, IP to detect Stat3 also known as Acute-phase response factor, DNA-binding protein APRF, signal transducer & activator of transcription 3.

Western Blot (SNAP ID) Analysis: 5 µg/mL from a representative lot detected STAT3 on 10 µg of HeLa cell lysate.

Immunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated STAT3 from 500 µg of HeLa RIPA lysate.

Biochem/physiol Actions

This antibody recognizes STAT3 near the C-terminus.

Physical form

Format: Purified

Protein A

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
HeLa cell lysate

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected STAT3 on 10 µg of HeLa cell lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Replaces: AB3162

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable