Belysa™ Analysis Software for Luminex xMAP® and SMCxPRO™ Platforms


 Belysa™ Analysis Software Version 1.0 Update and Documentation

 Cat. No. 40-122

Whether it’s multiplex, SMC™, or ELISA, Belysa™ Analysis Software is designed to give you confidence in your data. Belysa™ Analysis Software provides curve fitting capabilities with .csv output files from Luminex® xMAP® platforms, MAGPIX®, LX200 and FLEXMAP 3D® (FM3D), as well as the SMCxPRO™ ultrasensitive analyzer. Designed using current methods and principles, Belysa™ Analysis Software provides an easy-to-use environment to fit your data to the appropriate curve based on the individual calibrator profile within a multiplexed immunoassay. The software further examines for indicators of data hygiene to enhance your experimental integrity and reproducibility. Together the features of the package provide a user-friendly tool for data optimization before they use it to model a biological outcome.


Belysa™ Analysis Software Version 1.0 Key Features:

  • Curve fit optimization (4pl, 5pl, Linear, Cubic Spline)
  • Single and multi-analyte views
  • Robust auto-flagging and data hygiene features
  • Relative potency analysis for multiple compare curves
  • Modern look and feel
  • Flexible license mechanism. Activation dongle opens any copy.

System Requirements

  • PC with Microsoft Windows: 7 or higher
  • A free USB port on the USB
  • A Belysa™ license activation dongle

Explore unique ways to look closer at the data you generate by viewing
pre-recorded webinars

Belysa™: A Technical Seminar
Belysa™ software is designed to aid the end-user to understand the integrity of the raw data generated from their experiment. Here, we present the importance of data hygiene and analysis in an everchanging regulatory environment.  

Belysa Webinar Q&A   


Belysa™ Application Note

Gain more insight into your immunoassay methods with our Belysa™ Immunoassay Curve Fitting Software application note. Discover how to use the Belysa™ software’s mathematical parallelism function to compare calibration curves and confirm that your method was executed reproducibly either within the same batch of immunoassay or across multiple plates or lots.

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Visualize your calibration curve

The Belysa™ analysis software displays single and multiplex data that allows the user to immediately visualize their calibration curve and the distribution of standard points, controls and samples. Further information, derived from the curve, is provided along with the option to refit the curves through the Belysa™ software algorithms. Changes to interpolated data are instantly re-calculated following optimization (e.g. the slope equation, LLOQ or reported sample values).

Additionally, the detailed view within the software allows the parameters of the assay well to be observed. Two sets of identifiers allow users to draw attention to a potential deviation, a warning for software defined rules, such as Non-Detectable (ND) samples or user defined parameters (e.g. % recovery).


Quickly test the parallelism between different runs and compare calibration curves

For researchers looking to compare calibration curves, Belysa™ software provides the option to test the parallelism between different runs for the same assay. The assumption being that if the curves are relative then samples would report similarly if included on the other assay. A second metric of Relative Potency is also provided for those who wish to examine the consistency of a test material compared to a standard version.

Furthermore, Belysa™ software offers the option to visualize parallel curves with multiplex data and allows the user to quickly see if there are curves which do not have the expected relationship. The calculated value for this feature is conveniently presented in the lower window and reportable in an output file.

Taken together, these features provided by the Belysa™ software aid researchers in their ability to examine each individual assay and confirm that it was successfully executed. If the assay is performed as part of a multi-plate or longitudinal study, our Belysa™ software allows you to confirm the consistency of your method and that the data generated is free from experimental deviation which may affect your end results.