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Nerve Growth Factor-2.5S from murine submaxillary gland

Name: Nerve Growth Factor-2.5S from murine submaxillary gland
Brand: SIGMA
Price: 762 USD
Availability: InStock

lyophilized powder, suitable for cell culture

Synonym(s):

NGF-2.5S

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4 x 25 μg	Check Cart for Availability	$762.00

About This Item

CAS Number:

93928-24-6

UNSPSC Code:

12352202

NACRES:

NA.77

MDL number:

MFCD00161772

Biological source:

mouse

Form:

lyophilized powder

Mol wt:

26.5 kDa

$762.00

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Properties

Product Name

Nerve Growth Factor-2.5S from murine submaxillary gland, NGF-2.5S, lyophilized powder, suitable for cell culture

biological source

mouse

Quality Segment

200

form

lyophilized powder

potency

0.1-30 ng/mL EC₅₀ (using PC-12 cells)

mol wt

26.5 kDa

packaging

pkg of 4X25 μg

storage condition

avoid repeated freeze/thaw cycles

technique(s)

cell culture | mammalian: suitable

UniProt accession no.

P01139

shipped in

dry ice

storage temp.

−20°C

Gene Information

mouse ... Ngf(18049)

Description

General description

Nerve growth factor (NGF) is produced by different cell types: structural, accessory, and immune cells. It belongs to the neurotrophin family of trophic factors.

Application

Nerve Growth Factor-2.5S from murine submaxillary gland has been used:

in Ham′s F-12 medium for performing growth cone collapse assay using dorsal root ganglions (DRGs)
as a media supplement in the “process compartment” for microfluidic chamber studies
to treat mid-luteal cells to study its effect on choline acetyltransferase (CHAT) expression

Biochem/physiol Actions

Nerve growth factor (NGF) is neurotrophic and maintains sympathetic and sensory neurons of the peripheral nervous system and forebrain cholinergic neurons. NGF promotes the development of neurite-like filaments from chick embryo dorsal root ganglia and rat PC12 pheochromocytoma cells. NGF may have a role in in vivo fetal development and nerve regeneration especially, after ischemic, surgical, or chemical injuries. NGF may also be associated with the central nervous system. It exhibits ameliorating effects against ulcers (human cutaneous, corneal ulcers, and pressure ulcers), glaucoma, maculopathy, and retinitis pigmentosa. NGF-2.5S is a type of NGF-b originally derived under dissociative conditions and often varies from NGF-b due to proteolysis arising during its purification.

Physical form

Lyophilized from a 0.2 μm filtered solution in sodium acetate buffer.

Analysis Note

The biological activity is measured using a 3-day MTT assay.

Other Notes

2.5S subunit of nerve growth factor-7S (NGF-7S) is essentially the β-subunit when isolated from male mouse submaxillary glands under initially dissociative conditions by a modification of the method of Bocchini and Angeletti.

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This Item	N0513	P5739	F3133
Sigma-Aldrich N6009 Nerve Growth Factor-2.5S from murine submaxillary gland	Sigma-Aldrich N0513 Nerve Growth Factor-7S from murine submaxillary gland	Sigma-Aldrich P5739 Placenta Growth Factor-2 from mouse	Sigma-Aldrich F3133 Fibroblast Growth Factor from bovine pituitary
description NGF-2.5S, lyophilized powder, suitable for cell culture	description NGF-7S, lyophilized powder, suitable for cell culture	description lyophilized powder, recombinant, expressed in baculovirus infected Sf21 cells, suitable for cell culture	description suitable for cell culture
biological source mouse	biological source mouse	biological source mouse	biological source bovine pituitary glands
technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200
form lyophilized powder	form lyophilized powder	form lyophilized powder	form lyophilized powder
Gene Information mouse ... Ngf(18049)	Gene Information mouse ... Ngf(18049)	Gene Information mouse ... Pgf(18654)	Gene Information -
mol wt 26.5 kDa	mol wt protein 130 kDa	mol wt ~38 kDa by SDS-PAGE	mol wt 16.4 kDa

Storage Class

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, type N95 (US)

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