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R5158

RPMI-1640 Medium

HEPES modification with ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

RPMI culture medium, Roswell Park Memorial Institute 1640 medium

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Size/SKUAvailabilityPrice
500 mL

Available to ship TODAYfromMILWAUKEE

$56.30

About This Item

UNSPSC Code:
12352207
NACRES:
NA.71
MDL number:

$56.30


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Product Name

RPMI-1640 Medium, HEPES Modification, With 25 mM HEPES and L-glutamine, liquid, sterile-filtered, suitable for cell culture

Quality Segment

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

Endotoxin, tested

pH

>7.2

components

phenol red: 0.0053 g/L
L-glutamine: 0.3 g/L
NaHCO3: 2 g/L
HEPES: 5.96 g/L
glucose: 2 g/L (Dextro)

shipped in

ambient

storage temp.

2-8°C

General description

RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage-dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells. This medium is suitable for culturing human normal and neoplastic leukocytes.
This RPMI-1640 medium is supplemented with HEPES. HEPES is a Good′s physiological buffer that helps provide a more stable medium pH than bicarbonate buffers.

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description

HEPES Modification, With 25 mM HEPES and L-glutamine, liquid, sterile-filtered, suitable for cell culture

description

with 2.05 mM L-glutamine, with 25mM HEPES, liquid, sterile-filtered, suitable for cell culture

description

With L-glutamine and sodium bicarbonate. Without arginine, leucine, lysine, and phenol red, liquid, sterile-filtered, suitable for cell culture, designed for isotope labeling for cell culture applications

description

Hybri-Max, Modified, with L-glutamine, 4500 mg/L glucose and 15mM HEPES, without sodium bicarbonate, powder, suitable for hybridoma

sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

sterility

-

form

liquid

form

liquid

form

liquid

form

powder

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | hybridoma: suitable, cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | hybridoma: suitable

components

phenol red: 0.0053 g/L
L-glutamine: 0.3 g/L
NaHCO3: 2 g/L
HEPES: 5.96 g/L
glucose: 2 g/L (Dextro)

components

HEPES: 5958 mg/L
L-glutamine: 300 mg/L
NaHCO3: 2000 mg/L
phenol red: 5.310 mg/L

components

NaHCO3: 2 g/L
phenol red: no
glucose: 2 g/L (Dextro)
L-glutamine: 0.3 g/L

components

phenol red: 0.0053 g/L
L-glutamine: 0.3 g/L
HEPES: 3.5745 g/L
NaHCO3: no
glucose: 4.5 g/L (Dextro)

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C


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Storage Class

10 - Combustible liquids

WGK

WGK 2

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable



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