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SeqPlex RNA Amplification Kit

For use with high throughput sequencing technologies

Synonyme(s) :

WGA kit, Whole transcriptome amplification

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À vous/RéférenceDisponibilitéPrix
10 reactions
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989,00 €
50 reactions
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4 330,00 €
500 reactions
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22 650,00 €

A propos de cet article

NACRES:
NA.55
UNSPSC Code:
41121800

989,00 €


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Quality Segment

technique(s)

whole genome amplification: suitable

dilution

(WTA)

input

purified RNA

shipped in

wet ice

storage temp.

−20°C

General description

The SeqPlex RNA Amplification Kit for whole transcriptome amplification (WTA) is designed to facilitate next-generation sequencing (NGS) from small quantities or from degraded/highly fragmented RNA (e.g. RNA from formalin-fixed paraffin-embedded (FFPE) tissue samples). The SeqPlex kit allows the user to pre-amplify these and other small quantity/highly fragmented RNA samples for input into a NGS workflow. It also facilitates the amplification of non-polyA tailed RNA isolated from tissue, cultured cells, formalin-fixed samples, or serum while maintaining patterns of differential expression found in the unamplified sample.

Application

SeqPlex RNA Amplification Kit has been used to amplify poly(A)-selected RNA.[1][2]

Features and Benefits

  • Low quantities of total RNA random priming technology amplifies fragmented or intact RNA from all sources including FFPE and RIP.
  • Semi-degenerate library primer design for more complete transcriptome coverage and efficient priming.
  • No need to fragment DNA before sequencing.
  • Amplifies ds-cDNA in 8 hours or less.
  • Compatible with all next generation sequencing platforms except Pacific Bioscience.

Other Notes

SEQR-500RXN is manufactured on-demand. Contact us for more information.

Legal Information

SeqPlex is a trademark of Sigma-Aldrich Co. LLC

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Cet article
SEQXEWTA2WTA1
technique(s)

whole genome amplification: suitable

technique(s)

whole genome amplification: suitable

technique(s)

whole genome amplification: suitable

technique(s)

whole genome amplification: suitable

dilution

(WTA)

dilution

(WGA)

dilution

(WTA)

dilution

(WTA)

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

input

purified RNA

input

purified DNA

input

purified RNA

input

purified DNA

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200


Classe de stockage

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

wgk

WGK 3



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