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17-294

Rho Activation Assay Kit

PP1/PP2A Toolbox for the selective in vitro dephosphorylation of proteins.

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1 kit
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$1,130.00

About This Item

UNSPSC Code:
12161503
NACRES:
NA.84
eCl@ss:
32161000

$1,130.00

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Quality Segment

100

manufacturer/tradename

Upstate®

technique(s)

activity assay: suitable, affinity binding assay: suitable

NCBI accession no.

NM_001664.2

UniProt accession no.

P08134, P61586, P62745

detection method

chemiluminescent, colorimetric, fluorometric

shipped in

dry ice

Gene Information

human ... RHOA(387)

General description

A GST-tagged fusion protein, corresponding to residues 7-89 of mouse Rhotekin Rho Binding Domain, expressed in E. coli. Provided bound to glutathione-agarose. Purity >90%.

Application

PP1/PP2A Toolbox for the selective in vitro dephosphorylation of proteins.

Packaging

Kit capacity: 30 assays

Analysis Note

Routinely evaluated by precipitating GTP- Rho from 3T3/A31 cell lysates, subsequent detection by immunoblot analysis using anti-Rho (05-778).

Other Notes

100X GTPγS, 10mM (Cat.# 20-176)

100X GDP, 100mM (Cat.# 20-177)

Mg2+ Lysis/Wash Buffer, 5X (Cat.# 20-168)

Anti-Rho (-A, -B, -C), clone 55 (Cat.# 05-778)

Rho Assay Reagent (Rhotekin RBD, agarose) (Cat.# 14-383)

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item
143831445514278
Rho Activation Assay Kit PP1/PP2A Toolbox for the selective in vitro dephosphorylation of proteins.

Sigma-Aldrich

17294

Rho Activation Assay Kit

Rho Assay Reagent (Rhotekin RBD, agarose), 650 µg Specifically binds to & precipitates GTP-Rho, but not GDP-Rho from cell lysates. For use in Affinity Binding Assays.

Sigma-Aldrich

14383

Rho Assay Reagent (Rhotekin RBD, agarose), 650 µg

Rap1 Assay Reagent (Ral GDS-RBD, agarose), 650 µg Reagent (Ral GDS-RBD, agarose). A GST-tagged fusion protein, corresponding to amino acids 788-884 of human Ral GDS-Rap binding domain (RBD), for use in Affinity Binding Assays.

Sigma-Aldrich

14455

Rap1 Assay Reagent (Ral GDS-RBD, agarose), 650 µg

Ras Assay Reagent (Raf-1 RBD, agarose)

Sigma-Aldrich

14278

Ras Assay Reagent (Raf-1 RBD, agarose)

description

PP1/PP2A Toolbox for the selective in vitro dephosphorylation of proteins.

description

Specifically binds to & precipitates GTP-Rho, but not GDP-Rho from cell lysates. For use in Affinity Binding Assays.

description

Reagent (Ral GDS-RBD, agarose). A GST-tagged fusion protein, corresponding to amino acids 788-884 of human Ral GDS-Rap binding domain (RBD), for use in Affinity Binding Assays.

description

-

technique(s)

activity assay: suitable, affinity binding assay: suitable

technique(s)

affinity binding assay: suitable

technique(s)

affinity binding assay: suitable

technique(s)

activity assay: suitable

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

Gene Information

human ... RHOA(387)

Gene Information

mouse ... RTKN(20166)

Gene Information

human ... TERF2IP(54386)

Gene Information

human ... MRAS(22808)

NCBI accession no.

NM_001664.2

NCBI accession no.

NM_001015055.1, NM_001015056.1, NM_033046.2

NCBI accession no.

NM_018975.2

NCBI accession no.

NM_001085049.1, NM_012219.3

UniProt accession no.

P08134, P62745, P61586

UniProt accession no.

Q9BST9

UniProt accession no.

Q9NYB0

UniProt accession no.

O14807

manufacturer/tradename

Upstate®

manufacturer/tradename

Upstate®

manufacturer/tradename

Upstate®

manufacturer/tradename

Upstate®

pictograms

CorrosionEnvironment
GHS05,GHS09

signalword

Danger

Storage Class

10 - Combustible liquids

wgk

WGK 3

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 2 - ED ENV 1 - Eye Dam. 1

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