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14-383

Rho Assay Reagent (Rhotekin RBD, agarose), 650 µg

Specifically binds to & precipitates GTP-Rho, but not GDP-Rho from cell lysates. For use in Affinity Binding Assays.

Synonym(s):

Rho Assay Reagent, Rho Detection Reagent, Rhotekin RBD Assay Reagent

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650 μG

$662.00

$662.00

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About This Item

UNSPSC Code:
12352200
eCl@ss:
32160405
NACRES:
NA.84

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biological source

mouse

Quality Level

100

manufacturer/tradename

Upstate®

technique(s)

affinity binding assay: suitable

NCBI accession no.

NM_001015055.1
NM_001015056.1
NM_033046.2

UniProt accession no.

Q9BST9

shipped in

dry ice

Gene Information

mouse ... RTKN(20166)

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14-45514-41514-278
Rho Assay Reagent (Rhotekin RBD, agarose), 650 µg Specifically binds to & precipitates GTP-Rho, but not GDP-Rho from cell lysates. For use in Affinity Binding Assays.

Sigma-Aldrich

14-383

Rho Assay Reagent (Rhotekin RBD, agarose), 650 µg

Rap1 Assay Reagent (Ral GDS-RBD, agarose), 650 µg Reagent (Ral GDS-RBD, agarose). A GST-tagged fusion protein, corresponding to amino acids 788-884 of human Ral GDS-Rap binding domain (RBD), for use in Affinity Binding Assays.

Sigma-Aldrich

14-455

Rap1 Assay Reagent (Ral GDS-RBD, agarose), 650 µg

Ral Assay Reagent (Ral BP1, agarose)

Sigma-Aldrich

14-415

Ral Assay Reagent (Ral BP1, agarose)

Ras Assay Reagent (Raf-1 RBD, agarose)

Sigma-Aldrich

14-278

Ras Assay Reagent (Raf-1 RBD, agarose)

biological source

mouse

biological source

human

biological source

-

biological source

human

manufacturer/tradename

Upstate®

manufacturer/tradename

Upstate®

manufacturer/tradename

Upstate®

manufacturer/tradename

Upstate®

technique(s)

affinity binding assay: suitable

technique(s)

affinity binding assay: suitable

technique(s)

affinity binding assay: suitable

technique(s)

activity assay: suitable

NCBI accession no.

NM_001015055.1

NCBI accession no.

NM_018975.2

NCBI accession no.

NM_006788.3

NCBI accession no.

NM_001085049.1, NM_012219.3

UniProt accession no.

Q9BST9

UniProt accession no.

Q9NYB0

UniProt accession no.

Q15311

UniProt accession no.

O14807

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

General description

GST-tagged fusion protein, corresponding to residues 7-89 of mouse Rhotekin Rho Binding Domain, expressed in E.coli and bound to glutathione-agarose. Specifically binds to and precipitates GTP-Rho, but not GDP-Rho from cell lysates.
Product Source: Expressed in E. coli

Physical form

650 µg of recombinant protein in 1 mL glutathione-agarose slurry containing 45mM TRIS, pH 7.5, 0.45% Triton X-100, 135mM NaCl, 4.5mM MgCl2, 0.9mM dithiothreitol, 0.09mM PMSF, 0.9 µg/mL aprotinin, 0.9 µg/mL leupeptin, and 10% glycerol.

Preparation Note

Stable for 6 months at -70°C from date of receipt.

Analysis Note

Routinely evaluated by precipitating GTP-Rho from 3T3/A31 lysates, subsequent detection by immunoblot analysis using Anti-RhoA, clone 55 (05-778).

Other Notes

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

hcodes

H412

Hazard Classifications

Aquatic Chronic 3

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Determination of GTP loading on Rho.
X D Ren et al.
Methods in enzymology, 325, 264-272 (2000-10-19)
Simvastatin reduces human atrial myofibroblast proliferation independently of cholesterol lowering via inhibition of RhoA
Porter, K. E., et al
Cardiovascular Research, 61, 745-755 (2004)
Minji Jo et al.
The Journal of biological chemistry, 277(14), 12479-12485 (2002-01-24)
Binding of the urokinase-type plasminogen activator (uPA) to its receptor activates diverse cell signaling pathways. How these signals are integrated so that cell physiology is altered remains unclear. In this study, we demonstrated that migration of MCF-7 breast cancer cells
Geranylgeranyl diphosphate depletion inhibits breast cancer cell migration.
Amel Dudakovic,Huaxiang Tong,Raymond J Hohl
Investigational New Drugs null
Progestogens regulate endothelial actin cytoskeleton and cell movement via the actin-binding protein moesin.
Xiao-Dong Fu, Marina Flamini, Angel Matias Sanchez, Lorenzo Goglia, Maria Silvia Giretti et al.
Molecular Human Reproduction null
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