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537059

Propidium Iodide

Membrane impermeable DNA intercalator.

Synonym(s):

Propidium Iodide

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Size/SKUAvailabilityPrice
50 mg
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$132.00
100 mg
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$254.00
250 mg
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$589.00
1 g
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$1,970.00

About This Item

Empirical Formula (Hill Notation):
C27H34I2N4
CAS Number:
Molecular Weight:
668.39
UNSPSC Code:
12352200
NACRES:
NA.84
MDL number:
Form:
solid
Storage condition:
OK to freeze, protect from light

$132.00


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Quality Segment

description

RTECS - SF7949600

form

solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze, protect from light

color

dark red

input

sample type intact cells

fluorescence

λex ~536 nm, λem ~617 nm

shipped in

ambient

storage temp.

2-8°C

SMILES string

[I-].I.[N+](CCCN1C2=CC(=N)C=CC2=C3C(=C1c4ccccc4)C=C(C=C3)N)(CC)(CC)C

InChI

1S/C27H33N4.2HI/c1-4-31(3,5-2)17-9-16-30-26-19-22(29)13-15-24(26)23-14-12-21(28)18-25(23)27(30)20-10-7-6-8-11-20;;/h6-8,10-15,18-19,29H,4-5,9,16-17,28H2,1-3H3;2*1H/q+1;;/p-1

InChI key

XJMOSONTPMZWPB-UHFFFAOYSA-M

General description

Extinction coefficient(493 nm): 5900 M -1cm-1;(287 nm): 55,700 M -1cm-1;
Membrane impermeable DNA intercalator. Has red fluorescence at 488 nm. Useful for staining apoptotic cells and nuclei by flow cytometry. Can be used to differentiate between apoptotic and necrotic cell death. Can be used together with common green fluorescent probes labeled with fluorescein isothiocyanate. Has the following spectral properties: extinction coefficient: 5,900 M-1cm-1 (493 nm); 55,700 M-1cm-1 (287 nm); 13,800 M-1cm-1 (252.5 nm); excitation maximum: 536 nm; emission maximum: 617 nm.

Biochem/physiol Actions

Product does not compete with ATP.
Reversible: no
Primary Target
Membrane impermeable DNA intercalator
Cell permeable: no

Preparation Note

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Solubility may be improved by first dissolving in a very small amount of DMSO, then adding material to an aqueous solution.

Other Notes

Belloc, F., et al. 1994. Cytometry 17, 59.
Crompton, T., et al. 1992. Biochem. Biophys. Res. Commun. 183, 532.
Darzynkiewicz, Z., et al. 1992. Cytometry13, 795.
de Caestecker, M.P., et al. 1992. J. Immunol. Methods154, 11.
Pollice, A.A., et al. 1992. Cytometry13, 432.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Irritant (B)

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This Item
53706079214203989
description

Membrane impermeable DNA intercalator.

description

A convenient form of propidium iodide useful for flow cytometry studies.

description

1 mg/mL in H2O, ready to use solution, 100 assays

description

S-Butyrylthiocholine Iodide, CAS 1866-16-6, is a chromogenic substrate for cholinesterases. Its cleavage is measured colorimetrically at 405 nm.

form

solid

form

liquid

form

liquid

form

crystalline solid

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

200

manufacturer/tradename

Calbiochem®

manufacturer/tradename

Calbiochem®

manufacturer/tradename

-

manufacturer/tradename

Calbiochem®

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

storage condition

protect from light, OK to freeze

storage condition

OK to freeze, protect from light

storage condition

-

storage condition

OK to freeze, desiccated, protect from light

shipped in

ambient

shipped in

ambient

shipped in

-

shipped in

ambient


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pictograms

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signalword

Warning

hcodes

Hazard Classifications

Eye Irrit. 2 - Muta. 2

Storage Class

11 - Combustible Solids

flash_point_f

No data available

flash_point_c

No data available



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