The binding capacity of more than 85 nmol of free biotin per mL refers to 1 mL of settled agarose resin, not 1 mL of the 50% suspension. This product is supplied as a 50% slurry, meaning that 2 mL of suspension contains approximately 1 mL of settled resin. Therefore, 1 mL of suspension provides a binding capacity of approximately 42.5 nmol of free biotin or more.
69203
Streptavidin Agarose
Column or batch isolation of biotinylated molecules
Synonym(s):
Streptavidin beads
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| Size/SKU | Availability | Price |
|---|---|---|
5 mL | Check Cart for Availability | $476.00 |
About This Item
NACRES:
NA.56
UNSPSC Code:
41106500
Form:
liquid
Quality Segment
form
liquid
manufacturer/tradename
Novagen®
storage condition
do not freeze
technique(s)
biotinylated protein purification: suitable
shipped in
ambient
storage temp.
2-8°C
General description
| Specifications | |
| Concentration | 50% suspension (5 ml settled volume) |
| Storage buffer | 100 mM sodium phosphate pH 7.5, 0.02% sodium azide |
| Size | 40-165 µm |
| Binding capacity | >85 nmol free biotin/ml |
| Protease | None detected (SDS-PAGE of purified protein) |
| DNase | None detected (blue/white colony assay of ligated plasmid) |
| RNase | None detected (gel assay of purified RNA) |
Column or batch isolation of biotinylated molecules
Streptavidin Agarose consists of cross-linked agarose covalently coupled with pure streptavidin under controlled conditions. The stable linkage to the resin minimizes leaching of the streptavidin while maintaining full binding activity. The matrix is suitable for use in column and batch formats for any application that requires high biotin binding capacity and low non-specific binding. The resin has no detectable protease, DNase, or RNase contamination. Provided as a 50% slurry in storage buffer; 5 ml settled resin in 10 ml total volume.
Legal Information
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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This Item | |||
|---|---|---|---|
| description Column or batch isolation of biotinylated molecules | description suitable for affinity chromatography | description 5 mg Streptavidin for ELISA & Western Blotting. | description - |
| Quality Level 100 | Quality Level 100 | Quality Level 100 | Quality Level 300 |
| form liquid | form suspension | form - | form - |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. - | storage temp. - |
| manufacturer/tradename Novagen® | manufacturer/tradename - | manufacturer/tradename Chemicon® | manufacturer/tradename - |
| storage condition do not freeze | storage condition - | storage condition - | storage condition - |
| shipped in ambient | shipped in - | shipped in wet ice | shipped in - |
Storage Class
12 - Non Combustible Liquids
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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Does the binding capacity of > 85 nmol free biotin/ml refer to the 50% suspension or to the sedimented agarose?
1 answer-
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What is the recommended centrifuge speed for this product regarding biotinylated protein purification (especially washing steps)?
1 answer-
Centrifuge at 500 x g for 5 min.
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What is the total mass of the agarose in the 5ML pack size of product 69203-3, Streptavidin Agarose?
1 answer-
This product is provided as a 50% slurry, reflecting volume-to-volume ratio. The amount of streptavidin agarose used is strictly based on volume and not by its weight.
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