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8.51072

6-Carboxyfluorescein

Novabiochem®

Synonym(s):

6-Carboxyfluorescein, 6-FAM

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Size/SKUAvailabilityPrice
25 mg
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$59.30
100 mg
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$168.00

About This Item

Empirical Formula (Hill Notation):
C21H12O7
CAS Number:
Molecular Weight:
376.32
UNSPSC Code:
12352005
NACRES:
NA.22
MDL number:

$59.30


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Quality Segment

product line

Novabiochem®

assay

≥95% (TLC), ≥98.0% (HPLC)

form

powder

reaction suitability

reagent type: fluorescent-labelling reagent

manufacturer/tradename

Novabiochem®

application(s)

peptide synthesis

storage temp.

2-8°C

SMILES string

O1C3(c4c(cc(cc4)O)Oc5c3ccc(c5)O)c2c(ccc(c2)C(=O)O)C1=O

InChI

1S/C21H12O7/c22-11-2-5-14-17(8-11)27-18-9-12(23)3-6-15(18)21(14)16-7-10(19(24)25)1-4-13(16)20(26)28-21/h1-9,22-23H,(H,24,25)

InChI key

BZTDTCNHAFUJOG-UHFFFAOYSA-N

General description

This high purity fluorescent dye (λem494 nm, λex 518 nm) is supplied as a single isomer, which ensures production of labeled products of defined chemical structure, as well as greatly simplifying purification and characterization. FAM should be introduced as the last step in the synthesis to avoid formation of side-products, or protected by tritylation prior to acylation or hydrazine treatment [1].

Literature references

[1] R. Fischer, et al. (2003) Bioconjugate Chem., 14, 653.

Analysis Note

Color (visual): yellow to orange
Appearance of substance (visual): powder
Identity (IR): passes test
Purity (TLC (EtOAc/MeOH/H2O/DIPEA 60 : 30 : 10 : 3)): ≥ 95 %
Assay (HPLC, area%): ≥ 98.0 % (a/a)

To see the solvent systems used for TLC of Novabiochem® products please click here.

Other Notes

Replaces: 01-63-0113

Legal Information

Novabiochem is a registered trademark of Merck KGaA, Darmstadt, Germany

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application(s)

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application(s)

peptide synthesis

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form

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form

powder

Quality Level

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Quality Level

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Quality Level

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storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

-

assay

≥95% (TLC), ≥98.0% (HPLC)

assay

≥98% (TLC), ≥99.0% (HPLC)

assay

≥96% (HPLC)

assay

≥95% (HPLC)

product line

Novabiochem®

product line

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product line

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product line

BioReagent


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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable



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