Stericup® and Steritop® filters are fitted with a 1/4” tube adapter. We recommend 3/16 in. (4.8 mm) ID silicone tubing to fit this tube adapter: https://www.sigmaaldrich.com/product/mm/xx7100004
SCGPS02RE
Millipore® Steritop® Vacuum Bottle Top Filter
pore size 0.22 μm, polyethersulfone membrane, filter capacity 250 mL, 33 mm outlet thread(s), pack of 12 ea
Synonym(s):
Steritop Threaded Bottle Top Filter, Steritop®-GP Quick Release Bottle Top Filter, bottle top sterile filter unit, bottle top vacuum filter, bottle top vacuum filtering unit
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| Size/SKU | Availability | Price |
|---|---|---|
12 filters | Check Cart for Availability | $143.00 |
About This Item
UNSPSC Code:
41104914
NACRES:
NB.22
eCl@ss:
32031623
Pore size:
0.22 μm nominal pore size, 0.22 μm pore size
Filter capacity:
250 mL
Packaging:
pack of 12 ea
Material:
polyethersulfone membrane, polystyrene
Manufacturer/tradename:
Steritop®
Quality Segment
material
polyethersulfone membrane, polystyrene
product line
Steritop®
feature
holdup volume 2.4 mL, hydrophilic
packaging
pack of 12 ea
manufacturer/tradename
Steritop®
parameter
250 mL process volume, 250 mL sample volume, 45 °C max. temp.
technique(s)
sterile filtration: suitable
H
95 mm
diam.
12.5 cm
filter capacity
250 mL
filter diam.
33 mm
filtration area
40 cm2
matrix
Express PLUS™
pore size
0.22 μm nominal pore size, 0.22 μm pore size
fitting
inlet funnel, 33 mm outlet thread(s)
shipped in
ambient
storage temp.
15-25°C
General description
Work with ease and filter with confidence with Stericup® and Steritop® Filtration Systems.
Stericup ® and Steritop ® sterile filtration devices use reliable, high-performance membranes for optimum sterile filtration of laboratory reagents, buffers, and media. These devices combine superior flow rates and throughput with low non-specific binding and a stable, no-tip design.
This Steritop ® 250 mL bottle top 0.22 μm pore size polyethersulfone (PES) membrane filter unit can be used on bottles with 33 mm openings, , designed for vacuum applications.
Stericup ® and Steritop ® sterile filtration devices use reliable, high-performance membranes for optimum sterile filtration of laboratory reagents, buffers, and media. These devices combine superior flow rates and throughput with low non-specific binding and a stable, no-tip design.
This Steritop ® 250 mL bottle top 0.22 μm pore size polyethersulfone (PES) membrane filter unit can be used on bottles with 33 mm openings, , designed for vacuum applications.
Application
For Research Use Only
Stericup® and Steritop® systems are designed to reduce foaming and protein denaturation while maximizing flow and can be used for sterile vacuum filtration of aqueous solutions such as tissue culture media and biological fluids.
- Tissue culture media +/- additives
- Buffers
- Biological solutions
Legal Information
EXPRESS PLUS is a trademark of Merck KGaA, Darmstadt, Germany
Millipore is a registered trademark of Merck KGaA, Darmstadt, Germany
STERITOP is a registered trademark of Merck KGaA, Darmstadt, Germany
1 of 1
This Item | |||
|---|---|---|---|
| description pore size 0.22 μm, polyethersulfone membrane, filter capacity 250 mL, 33 mm outlet thread(s), pack of 12 ea | description pore size 0.22 μm, polyethersulfone membrane, filter capacity 150 mL, 33 mm outlet thread(s), pack of 12 ea | description pore size 0.22 μm, polyethersulfone membrane, filter capacity 500 mL, 33 mm outlet thread(s), pack of 12 ea | description pore size 0.1 μm, polyethersulfone membrane, filter capacity 1000 mL, pack of 12 ea |
| manufacturer/tradename Steritop® | manufacturer/tradename - | manufacturer/tradename - | manufacturer/tradename - |
| filter capacity 250 mL | filter capacity 150 mL | filter capacity 500 mL | filter capacity 1000 mL |
| pore size 0.22 μm pore size, 0.22 μm nominal pore size | pore size 0.22 μm pore size | pore size 0.22 μm pore size | pore size 0.1 μm pore size |
| material polystyrene , polyethersulfone membrane | material polyethersulfone membrane, polystyrene funnel(s), polystyrene housing | material polyethersulfone membrane, polystyrene funnel(s), polystyrene housing | material polyethersulfone membrane, polystyrene funnel(s), polystyrene housing, polystyrene bottle |
| feature holdup volume 2.4 mL, hydrophilic | feature hydrophilic | feature hydrophilic | feature - |
| packaging pack of 12 ea | packaging pack of 12 ea | packaging pack of 12 ea | packaging pack of 12 ea |
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What tubing can be used with Stericup® and Steritop® vacuum filters?
What tubing can be used with Stericup® and Steritop® vacuum filters?
1 answer-
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What is the maximum vacuum pressure for filtration?
1 answer-
700 mm Hg (27.5” Hg, 0.9 bar) differential vacuum at 25 °C (77 °F)
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What is the maximum processing volume for Stericup® and Steritop® filters? Can I add more volume after initial filtration?
1 answer-
All Stericup® and Steritop® filters were developed for single-use application. The maximum volume is dependent on the receiver bottle capacity. Stericup® filters come with a receiver bottle with a specified maximum volume. For Steritop® filters, the maximum recommended volume is 2 L with an appropriate-sized receiver bottle. Larger processing volumes can result in membrane fouling, increase chance of spillage and contamination, and lead to possible airlock*.
*Note that devices with PES membranes can airlock if filtered to dryness. Some liquid must be left on top of the filter, then the system stopped, receiver changed or emptied, and more sample volume added before restart. This increases the chance of spillage.Helpful?
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What leachables are detected during filtration processes using Stericup® filters?
1 answer-
We have not performed extractables/leachables studies for our Stericup® filters.
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Are Stericup® and Steritop® filters GMP-certified?
1 answer-
No, Stericup® and Steritop® devices are not GMP-approved.
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The User Guide says “Use only glass or plastic bottles designed for vacuum applications”. Can you specify which bottles are compatible?
1 answer-
Steritop® Quick Release bottle top units require a vacuum-rated receiver bottle with either a GL33 or GL45 mm closure thread as specified. Steritop® E bottle top units require a vacuum-rated receiver bottle with a GL45 mm closure thread. Bottles no larger than 2 L should be used.
Glass bottles, GL33:
• Wheaton media bottles (glass), 500 mL (Product Z364886)
• Wheaton media bottles (glass), 1 L (Product Z364894)
Glass bottles, GL45
• Corning® glass media storage bottle, 125 mL (Product CLS1399125)
• Corning® glass media storage bottle, 250 mL (Product CLS1399250)
• Corning® glass media storage bottle, 500 mL (Product CLS1399500)
• Corning® glass media storage bottle, 1 L (Product CLS13991L)
• Pyrex® round media storage bottle, 500 mL (Product CLS1395500)
• Pyrex® round media storage bottle, 1 L (Product CLS13951L)
• KIMBLE® KIMAX® Media bottle, 250 mL (Product DWK14395250)
• KIMBLE® KIMAX® Media bottle, 500 mL (Product DWK14395500)
• KIMBLE® KIMAX® Media bottle, 1 L (Product DWK143951000)
Plastic (pre-sterile) bottles, GL45:
• Stericup® Quick Release Receiver flask, 250 mL (Product S200B02RE)
• Stericup® Quick Release Receiver flask, 500 mL (Product S200B05RE)
• Stericup® Quick Release Receiver flask, 1000 mL (Product S200B10RE)Helpful?
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Which type of pump should be used with Stericup® and Steritop® vacuum filters?
Which type of pump should be used with Stericup® and Steritop® vacuum filters?
1 answer-
We recommend our Chemical Duty Pump: https://www.sigmaaldrich.com/product/mm/wp6111560
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What is the chemical compatibility of the various Stericup® and Steritop® filters?
What is the chemical compatibility of the various Stericup® and Steritop® filters?
1 answer-
Please refer to our guide, "Chemical compatibility of filter components": https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/302/855/membrane-chemical-compatibility-ds2532en-ms.pdf
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Can Stericup® and Steritop® filters be reused?
1 answer-
All Stericup® and Steritop® filters were developed for single use, disposable processing.
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Can we filter oil-type liquids with Stericup® filters?
Can we filter oil-type liquids with Stericup® filters?
1 answer-
Likely not, due to pore size/possible fouling/chemical compatibility. Refer to our guide, "Chemical compatibility of filter components": https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/302/855/membrane-chemical-compatibility-ds2532en-ms.pdf
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