UFC200324
Amicon® Ultra Centrifugal Filter, 3 kDa MWCO
sample volume 2 mL, regenerated cellulose membrane, MWCO 3 kDa
Sustainability features
Synonym(s):
Centrifugal concentrator, 3 kDa MWCO, 2 mL sample volume
Select a Size
| Size/SKU | Availability | Price |
|---|---|---|
24 pkg | Check Cart for Availability | $215.00 |
About This Item
$215.00
Quality Segment
material
polypropylene tube (for collection), regenerated cellulose membrane, styrene-butadiene housing
sterility
non-sterile
product line
Amicon®
packaging
pkg of 24 pkg
manufacturer/tradename
Amicon® Ultra
sustainability
Sustainability features
parameter
2 mL sample volume
technique(s)
Diafiltration: suitable, protein extraction: suitable, protein purification: suitable
L
119.7 mm
diam.
15.9 mm
filtration area
1 cm2
volume
2 mL
working volume
≤2 mL , 2 mL
pore size
3 kDa MWCO
shipped in
ambient
General description
The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 50-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss. Efficient recovery of the concentrated sample (retained species) is achieved by a convenient reverse spin step after collecting the filtrate.
The device can be spun in a swinging bucket or fixed angle rotor. Amicon® Ultra-2 mL devices are supplied non-sterile and are for single use only. With their vertical membrane design and reverse spin, Amicon® Ultra 2 mL filters deliver the highest recovery with shortest spin times.
Application
- Ultrafiltration with 3,000 Dalton molecular weight cutoff Ultracel® membrane.
- Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms.
- Purification of macromolecular components found in tissue culture extracts or cell lysates.
- Primer removal, PCR cleanup, removal of linkers or macromolecular labels from a reaction mix, and protein removal prior to HPLC.
- Desalting, buffer exchange and protein dialysis.
- Size exclusion and protein fractionation.
Features and Benefits
- Ultracel® regenerated cellulose membrane with 3 kDa NMWCO delivers retentate recovery of greater than 90%
- Concentrates 2 mL down to 15-70 μL
- 98% sample recovery
- Fast processing time: 10 to 30-minute spin times
- Engineered dead stop
- Reverse spin capability provides consistent recoveries
Physical form
Other Notes
Legal Information
1 of 1
This Item | |||
|---|---|---|---|
| description sample volume 2 mL, regenerated cellulose membrane, MWCO 3 kDa | description sample volume 15 mL, regenerated cellulose membrane, MWCO 3 kDa | description sample volume 0.5 mL, regenerated cellulose membrane, MWCO 3 kDa | description sample volume 4 mL, regenerated cellulose membrane, MWCO 3 kDa |
| pore size 3 kDa MWCO | pore size 3 kDa MWCO | pore size 3 kDa MWCO | pore size 3 kDa MWCO |
| sterility non-sterile | sterility non-sterile | sterility non-sterile | sterility non-sterile |
| parameter 2 mL sample volume | parameter 15 mL sample volume | parameter 0.5 mL sample volume | parameter 4 mL sample volume |
| manufacturer/tradename Amicon® Ultra | manufacturer/tradename Amicon® Ultra | manufacturer/tradename Amicon® Ultra | manufacturer/tradename Amicon® Ultra |
| material polypropylene tube (for collection), styrene-butadiene housing, regenerated cellulose membrane | material regenerated cellulose membrane, polyethylene liner, polyethylene cap, regenerated cellulose support, styrene-butadiene housing, polypropylene tube | material polypropylene tube, regenerated cellulose membrane, polypropylene cap, styrene-butadiene housing | material styrene-butadiene housing, regenerated cellulose support, polyethylene cap, polypropylene tube, polyethylene liner, regenerated cellulose membrane |
| working volume ≤2 mL | working volume ≤15 mL | working volume 0.5 mL , ≤500 μL | working volume ≤4 mL |
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