The Amicon Ultra devices are non-sterile. However, many customers rinse with 70% IPA then discard, let dry, then introduce a sample to the product.
UFC8003
Amicon® Ultra Centrifugal Filter, 3 kDa MWCO
sample volume 4 mL, regenerated cellulose membrane, MWCO 3 kDa
Sustainability features
Synonym(s):
Centrifugal concentrator, 3 kDa MWCO, 4 mL sample volume
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| Size/SKU | Availability | Price |
|---|---|---|
8 pkg | Check Cart for Availability | $86.30 |
24 pkg | Check Cart for Availability | $210.00 |
96 pkg | Check Cart for Availability | $795.00 |
About This Item
UNSPSC Code:
41104916
NACRES:
NB.24
eCl@ss:
36100101
Material:
polyethylene cap, polyethylene liner, polypropylene tube, regenerated cellulose membrane, regenerated cellulose support, styrene-butadiene housing
Pore size:
3 kDa MWCO
Working volume:
≤4 mL , 4 mL
Sterility:
non-sterile
Packaging:
pkg of 24 pkg, pkg of 8 pkg, pkg of 96 pkg
Quality Segment
material
polyethylene cap, polyethylene liner, polypropylene tube, regenerated cellulose membrane, regenerated cellulose support, styrene-butadiene housing
sterility
non-sterile
product line
Amicon®
packaging
pkg of 24 pkg, pkg of 8 pkg, pkg of 96 pkg
manufacturer/tradename
Amicon® Ultra
sustainability
Sustainability features
parameter
4 mL sample volume
technique(s)
Diafiltration: suitable, protein extraction: suitable, protein purification: suitable
L
124 mm (4.88 in.)
diam.
17.3 mm
filtration area
3 cm2
volume
4 mL
working volume
≤4 mL , 4 mL
pore size
3 kDa MWCO
shipped in
ambient
General description
Amicon® Ultra 4 mL centrifugal devices with Ultracel® 3,000 Dalton molecular weight cutoff filters provide fast ultrafiltration, and reliable retention of macromolecules. For example, Cytochrome c (12.4 kDa) at 0.25 mg/ml initial concentration is retained at >95% .
The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 80-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss.
The concentrate is collected from the filter device sample reservoir using a pipetter, while the ultrafiltrate is collected in the provided centrifuge tube. The device can be spun in a swinging bucket or fixed angle rotor. With their vertical membrane design and deadstop, Amicon® Ultra 15 mL filters deliver the highest recovery with short spin times.
The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 80-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss.
The concentrate is collected from the filter device sample reservoir using a pipetter, while the ultrafiltrate is collected in the provided centrifuge tube. The device can be spun in a swinging bucket or fixed angle rotor. With their vertical membrane design and deadstop, Amicon® Ultra 15 mL filters deliver the highest recovery with short spin times.
We are committed to bringing you Greener Alternative Products, which have been re-engineered to enable more sustainable research solutions. The inner filtration device is made from 60% bio-circular material allocated using the ISCC mass balance approach. Click here for more information.
Application
- Ultrafiltration with 3,000 Dalton molecular weight cutoff Ultracel® membrane.
- Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms.
- Purification of macromolecular components found in tissue culture extracts or cell lysates and protein removal prior to HPLC.
- Concentration of dilute or pre-purified proteins from column eluents.
- Desalting, buffer exchange and protein dialysis.
- Size exclusion and protein fractionation.
Features and Benefits
- Ultracel® regenerated cellulose membrane with 3 kDa NMWCO delivers retentate recovery of greater than 90%.
- Vertical membrane reduces concentration polarization for ultra-fast spin times (as fast as 5 minutes).
- Heat-sealed membrane minimizes downstream extractables.
- 100% integrity tested for reliable performance.
- Convenient sample monitoring with translucent housing and volume gradations.
- Direct pipette sample access eliminates processing step to recover concentrate.
- High concentration factors of 80–100X.
Physical form
Color Code: Clear with pink cap
Legal Information
Amicon is a registered trademark of Merck KGaA, Darmstadt, Germany
ULTRACEL is a registered trademark of Merck KGaA, Darmstadt, Germany
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This Item | |||
|---|---|---|---|
| description sample volume 4 mL, regenerated cellulose membrane, MWCO 3 kDa | description sample volume 2 mL, regenerated cellulose membrane, MWCO 3 kDa | description sample volume 0.5 mL, regenerated cellulose membrane, MWCO 3 kDa | description sample volume 15 mL, regenerated cellulose membrane, MWCO 3 kDa |
| manufacturer/tradename Amicon® Ultra | manufacturer/tradename Amicon® Ultra | manufacturer/tradename Amicon® Ultra | manufacturer/tradename Amicon® Ultra |
| pore size 3 kDa MWCO | pore size 3 kDa MWCO | pore size 3 kDa MWCO | pore size 3 kDa MWCO |
| parameter 4 mL sample volume | parameter 2 mL sample volume | parameter 0.5 mL sample volume | parameter 15 mL sample volume |
| sterility non-sterile | sterility non-sterile | sterility non-sterile | sterility non-sterile |
| material styrene-butadiene housing, regenerated cellulose support, polyethylene cap, polypropylene tube, polyethylene liner, regenerated cellulose membrane | material polypropylene tube (for collection), styrene-butadiene housing, regenerated cellulose membrane | material polypropylene tube, regenerated cellulose membrane, polypropylene cap, styrene-butadiene housing | material regenerated cellulose membrane, polyethylene liner, polyethylene cap, regenerated cellulose support, styrene-butadiene housing, polypropylene tube |
| working volume ≤4 mL | working volume ≤2 mL | working volume 0.5 mL , ≤500 μL | working volume ≤15 mL |
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Good day, I would like to know if these concentrators are sterile and the recovered proteins are suitable for downstream cell culture assays. Thank you!
1 answer-
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What could be the reason for no protein recovery (in the concentrate or flow through) while using the UFC800324 to purify protein? Could the protein may be absorbed in the filter?
1 answer-
The low or no protein recovery issue may be attributed to over-concentration of the protein, leading to precipitation. The User Guide provides guidance on maximizing sample recovery: Low sample recovery in the concentrate may be due to adsorptive losses, over-concentration, or passage of sample through the membrane. Adsorptive losses depend upon solute concentration, its hydrophobic nature, temperature and time of contact with filter device surfaces, sample composition, and pH. To minimize losses, remove concentrated samples immediately after centrifugal spin. If the starting sample concentration is high, monitor the centrifugation process in order to avoid over-concentration of the sample. Over-concentration can lead to precipitation and potential sample loss. If the sample appears to be passing through the membrane, choose a lower MWCO Amicon device.
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What is max g force these filters can take? i.e. what sort of rpm or g force should I spin at?
1 answer-
The UFC8003 protocol states to spin at 7,500 × g maximum for approximately 10–40 minutes. See the image below for further details.
Helpful?
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Can we centrifuge these tubes in a normal 15 mL swinging bucket centrifuge?
1 answer-
Yes, the device can be used in centrifuges with a swinging bucket or fixed-angle rotor.
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