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11699105001

Roche

PCR Buffer Without MgCl2, 10x concentrated

solution, pkg of 3 × 1 mL

Synonym(s):

buffer

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3 x 1 mL
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$76.40

About This Item

UNSPSC Code:
41105600
NACRES:
NA.54

$76.40

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form

solution

packaging

pkg of 3 × 1 mL

manufacturer/tradename

Roche

technique(s)

PCR: suitable, whole genome amplification: suitable

storage temp.

−20°C

General description

The PCR buffer without MgCl2, in combination with the MgCl2 stock solution, is used for the individual adjustment of the Mg2+ concentration in the PCR reaction. In most applications a concentration of 1.5mM MgCl2 will yield satisfactory results with a dNTP concentration of 200μM each. In many cases, however, it is required to titrate the optimal Mg2+ concentration to increase the specificity and yield of the PCR reaction.The buffer is ten-fold concentrated and should be used in combination with the MgCl2 stock solution.

Application

The PCR buffer without MgCl2 has been used for the amplification of specific DNA fragments by the polymerase chain reaction (PCR).

Physical form

Solution, 100 mM Tris-HCl, 500 mM KCl (pH 8.3 at 20 °C)

Other Notes

For general laboratory use.

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This Item
P2317P2192M9943
PCR Buffer Without MgCl2, 10x concentrated solution, pkg of 3 × 1 mL

Roche

11699105001

PCR Buffer Without MgCl2, 10x concentrated

10X PCR Buffer without MgCl2 Optimized for routine PCR without MgCl2

Sigma-Aldrich

P2317

10X PCR Buffer without MgCl2

10X PCR Buffer Optimized for routine PCR with MgCl2 included

Sigma-Aldrich

P2192

10X PCR Buffer

10X MTP™ Taq Buffer 10X buffer, free of DNA contaminants; use with MTP Taq DNA Polymerase

Sigma-Aldrich

M9943

10X MTP™ Taq Buffer

description

solution, pkg of 3 × 1 mL

description

Optimized for routine PCR without MgCl2

description

Optimized for routine PCR with MgCl2 included

description

10X buffer, free of DNA contaminants; use with MTP Taq DNA Polymerase

technique(s)

whole genome amplification: suitable, PCR: suitable

technique(s)

PCR: suitable, whole genome amplification: suitable

technique(s)

whole genome amplification: suitable, PCR: suitable

technique(s)

whole genome amplification: suitable, PCR: suitable

form

solution

form

liquid

form

liquid

form

liquid

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

packaging

pkg of 3 × 1 mL

packaging

-

packaging

vial of 1.5 mL

packaging

-

manufacturer/tradename

Roche

manufacturer/tradename

-

manufacturer/tradename

-

manufacturer/tradename

-

Storage Class

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point (°F)

does not flash

Flash Point (°C)

does not flash

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