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BCIPP-RO

Roche

BCIP

99% (HPLC), powder, solubility: in DMF, soluble

Synonym(s):

5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt, BCIP® p-toluidine salt, X-phosphate p-toluidine salt

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250 MG

$227.00

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$494.00

$227.00

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About This Item

Empirical Formula (Hill Notation):
C8H6BrClNO4P · C7H9N
CAS Number:
6578-06-9
Molecular Weight:
433.62
UNSPSC Code:
12352204
PubChem Substance ID:
329774194
MDL number:
MFCD00040636
NACRES:
NA.54

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description

5-bromo-4-chloro-3-indolyl-phosphate, 4-toluidine salt

assay

99% (HPLC)

form

powder

mol wt

Mr 326.44 (BCIP), Mr 433.6 (BCIP toluidine salt)

packaging

pkg of 1 g (11585002001), pkg of 250 mg (10760994001)

manufacturer/tradename

Roche

solubility

DMF: soluble

storage temp.

2-8°C

SMILES string

Cc1ccc(N)cc1.OP(O)(=O)Oc2c[nH]c3ccc(Br)c(Cl)c23

InChI

1S/C8H6BrClNO4P.C7H9N/c9-4-1-2-5-7(8(4)10)6(3-11-5)15-16(12,13)14;1-6-2-4-7(8)5-3-6/h1-3,11H,(H2,12,13,14);2-5H,8H2,1H3

InChI key

QEIFSLUFHRCVQL-UHFFFAOYSA-N

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This Item
1168145100111383221001HPA013562
BCIP 99% (HPLC), powder, solubility: in DMF, soluble

Roche

BCIPP-RO

BCIP

NBT/BCIP Stock Solution

Roche

11681451001

NBT/BCIP Stock Solution

BCIP solution, >95% (HPLC), pkg of 3 mL (150 mg)

Roche

11383221001

BCIP

Anti-OPN1SW antibody produced in rabbit Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody

Sigma-Aldrich

HPA013562

Anti-OPN1SW antibody produced in rabbit

assay

99% (HPLC)

assay

-

assay

>95% (HPLC)

assay

-

solubility

DMF: soluble

solubility

-

solubility

-

solubility

-

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

form

powder

form

solution

form

solution

form

buffered aqueous glycerol solution

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

description

5-bromo-4-chloro-3-indolyl-phosphate, 4-toluidine salt

description

-

description

4-toluidine salt

description

-

General description

BCIP serves as substrate for alkaline phosphatase. The reaction product has a blue color and is insoluble in water. The blue color can be visually detected.
Histochemical substrate for alkaline phosphatase.

Application

For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry.
BCIP is provided in two salt forms: the disodium salt which is soluble in water and the p-toluidine form which is soluble in dimethylformamide. These salt forms may be used to prepare a stock solution that in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase. This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color. For added convenience, a mixture of BCIP and NBT is provided in an easily dissolvable tablet form or as a ready-to-use liquid.
For the colorimetric detection of alkaline phosphatase-labeled molecules.

Analysis Note

Performance test: incubation with AP
Absorption: Absorption maximum in the visible light is not determined.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Legal Information

BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

Exclamation mark
GHS07

signalword

Warning

Hazard Classifications

Acute Tox. 4 Dermal - Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk

WGK 2

flash_point_f

136.4 °F

flash_point_c

58 °C

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Certificates of Analysis (COA)

Lot/Batch Number
55741422
42998525
42998523
42998521
40755324

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David G Simmons et al.
Developmental biology, 304(2), 567-578 (2007-02-10)
Trophoblast giant cells (TGCs) are the first terminally differentiated subtype to form in the trophoblast cell lineage in rodents. In addition to mediating implantation, they are the main endocrine cells of the placenta, producing several hormones which regulate the maternal

Global Trade Item Number

SKUGTIN
1158500200104061838251077
1076099400104061838675408

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