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DGTP-RO

Roche

dGTP

Name: dGTP
Brand: ROCHE

PCR Grade, sodium salt

Synonyme(s) :

2′-Deoxyguanosine 5′-triphosphate trisodium salt solution, dGTP-Na₃

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A propos de cet article

Formule empirique (notation de Hill) :

C₁₀H₁₃N₅Na₃O₁₃P₃

Numéro CAS:

93919-41-6

Poids moléculaire :

573.13

UNSPSC Code:

12352302

PubChem Substance ID:

329798919

MDL number:

MFCD00080337

NACRES:

NA.54

Assay:

99% (dNTP)

Form:

solution

Storage temp.:

−20°C

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Propriétés

assay

99% (dNTP)

form

solution

usage

sufficient for 125,000 standard reactions (03732703001), sufficient for 31,250 standard reactions (11969030001), sufficient for 6,250 standard reactions (11934538001)

packaging

pkg of 1,250 μL (11969030001 [100 mM]), pkg of 4 × 1,240 μL (03732703001 [4x100 mM]), pkg of 250 μL (11934538001 [100 mM])

manufacturer/tradename

Roche

concentration

<0.9% (dNDP), 100 mM

storage temp.

−20°C

SMILES string

[Na+].[Na+].[Na+].NC1=Nc2c(ncn2[C@H]3C[C@H](O)[C@@H](COP([O-])(=O)OP([O-])(=O)OP(O)([O-])=O)O3)C(=O)N1

InChI

1S/C10H16N5O13P3.3Na/c11-10-13-8-7(9(17)14-10)12-3-15(8)6-1-4(16)5(26-6)2-25-30(21,22)28-31(23,24)27-29(18,19)20;;;/h3-6,16H,1-2H2,(H,21,22)(H,23,24)(H2,18,19,20)(H3,11,13,14,17);;;/q;3*+1/p-3/t4-,5+,6+;;;/m0.../s1

InChI key

IWGGLKOTEOCWQP-BIHLCPNHSA-K

Description

General description

2′-Deoxyguanosine 5′-triphosphate (dGTP) is made of a guanine nucleobase attached to deoxyribose and a 5′-hydroxyl on the sugar linked to a chain of three phosphate residues. It is used by the cells for the synthesis of DNA with the help of DNA polymerase.

dGTP, PCR Grade, is a 100 mM clear, colorless solution of the sodium salt (pH 8.3). PCR Grade nucleotides from Roche are specially manufactured and purified to the highest possible chemical purity. PCR Grade nucleotides contain at least 99% of the relevant deoxynucleoside-triphosphate (dNTP) and less than 0.9% deoxynucleoside-diphosphate (dNDP).

Application

2′-Deoxyguanosine 5′-triphosphate trisodium salt is utilized for methods such as DNA sequencing, PCR and other DNA polymerase based DNA synthesis and repair techniques.

Physical form

solution of dGTP in water (pH 8.3).

Preparation Note

Working solution: We recommend to prepare a deoxynucleotide mix containing dATP, dCTP, dGTP, dTTP (10 mM, each); (e.g., for the preparation of 100 μl nucleotide mix add 10 μl of dATP, dCTP, dGTP, dTTP (each) to 60 μl Water, PCR Grade).

Analysis Note

Function tested in RT-PCR. Tested for absence of contaminating deoxyribonucleases, ribonucleases and nicking activities according to the current Quality Control procedures.

Other Notes

For general laboratory use.

Comparer avec des articles similaires

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1 of 1

Cet article	DNTP-RO	11051466001	DNSTP-RO
Roche DGTP-RO dGTP	Roche DNTP-RO Lot de désoxynucléoside-triphosphates	Roche 11051466001 dGTP	Roche DNSTP-RO Deoxynucleoside Triphosphate Set
manufacturer/tradename Roche	manufacturer/tradename Roche	manufacturer/tradename Roche	manufacturer/tradename Roche
assay 99% (dNTP)	assay 99% (dNTP)	assay 98%	assay ≥98% (dntp)
form solution	form liquid	form solution	form liquid
packaging pkg of 1,250 μL (11969030001 [100 mM]), pkg of 250 μL (11934538001 [100 mM]), pkg of 4 × 1,240 μL (03732703001 [4x100 mM])	packaging pkg of 4 × 1,250 μL (03622614001 [4x100 mM]), pkg of 4 × 250 μL (11969064001 [4x100 mM])	packaging pkg of 250 μL (25 μmol; 100 mM)	packaging pkg of 4 × 100 μL (11277049001 [4x10 μmol]), pkg of 40 × 100 μL (11922505001 [40x100mM])
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C
concentration <0.9% (dNDP), 100 mM	concentration <0.9% (dNDP)	concentration 100 mM	concentration -

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