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FastStart Universal Probe Master (Rox)

Name: FastStart Universal Probe Master (Rox)
Brand: ROCHE
Price: 216 USD
Availability: InStock

sufficient for 250 reactions, sufficient for 1250 reactions, sufficient for 5000 reactions, suitable for qPCR, suitable for RT-qPCR

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Size/SKU	Availability	Price
250 x 20 reactions	Check Cart for Availability	$216.00
1250 x 20 reactions	Check Cart for Availability	$1,010.00 $888.80
5000 x 20 reactions	Check Cart for Availability	$3,640.00

About This Item

NACRES:

NA.55

UNSPSC Code:

41106300

$216.00

Check Cart for Availability

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Properties

usage

sufficient for 1250 reactions, sufficient for 250 reactions, sufficient for 5000 reactions

feature

dNTPs included: no, hotstart

manufacturer/tradename

Roche

packaging

pkg of 1250 x 20 μL reactions (04913957001), pkg of 250 x 20 μL reactions (04913949001), pkg of 5000 x 20 μL reactions (04914058001)

technique(s)

RT-qPCR: suitable, qPCR: suitable, whole genome amplification: suitable

input

purified DNA

detection method

probe-based

Description

General description

Hot start protocols have been shown to significantly improve the specificity, sensitivity, and yield of PCR. Heat-labile blocking groups on some of the amino acid residues of FastStart^™ Taq DNA Polymerase make the modified enzyme inactive at room temperature. Therefore, there is no elongation during the period when primers can nonspecifically bind. FastStart^™ Taq DNA Polymerase is activated by removing the blocking groups at a high temperature (i.e., a preincubation step at +95°C).

Universal ready-to-use hot start reaction mix for qPCR and RT-qPCR on all real-time PCR systems requiring normalization with ROX.

FastStart^™ Universal Probe Master (Rox) includes a novel reference dye that enables its use on all real-time PCR instruments requiring normalization with ROX, without modification or adjustments to the specific instrument or protocol. This ready-to-use, 2x concentrated master mix contains all reagents (except primers, probe, and template) needed for running quantitative, real-time DNA-detection assays, including qPCR and two-step qRT-PCR, in the hydrolysis probe detection format. FastStart^™ Universal Probe Master (Rox) generates excellent results on instruments such as the Applied Biosystems 7900 HT Fast Real-Time PCR System or the Applied Biosystems 7500 Real-Time PCR System. This product is not intended for use with the LightCycler^® Instruments.

Application

FastStart^™ Universal Probe Master (Rox) has been used:

in TaqMan^® quantitative real-time polymerase chain reaction (qRT-PCR) reactions for the quantification of endogenous miRNAs, such as MIR376B, MIR376A and MIR181A
in reverse transcriptase (RT-PCR) to study tumor necrosis factor (TNF) expression in whole synovial tissue of undifferentiated peripheral inflammatory arthritis (UPIA) patients
for the amplification and detection of any DNA or cDNA target, including those that are GC- or AT-rich by quantitativePCR

Combine this master mix with Transcriptor First Strand cDNA Synthesis Kit (Roche) to achieve excellent results in two-step qRT-PCR.

Features and Benefits

Increase qPCR sensitivity and specificity.

Produce lower cycle threshold (Ct) values.

Use the master mix with any probe-based assay.

Achieve sensitive, specific results in assays with the Universal ProbeLibrary Probes or any other hydrolysis probe.

Amplify and detect a broad range of DNA or cDNA targets.

Amplify fragments up to 500 bp long, including those that are GC- or AT-rich.

Visualize amplification products on agarose gels.

Use robotic pipetting stations to set up qPCR reactions.

Use a master mix that is stable at room temperature during extended reaction setup times.

Prevent false positives resulting from carryover contamination.

Use this dUTP-containing mix with Uracil-DNA Glycosylase to eliminate contaminating DNA carried over from previous PCR reactions.

Analysis Note

Function test: Each lot is tested for performance in qPCR using three templates: a GC–rich template, an AT-rich template, and a long template (approximately 440 bp).

Other Notes

FastStart Universal Probe Master (Rox), 2x concentrated master mix that contains FastStart Taq DNA Polymerase, Reaction Buffer, Nucleotides (dATP, dCTP, dGTP, dUTP), and a reference dye.

For life science research only. Not for use in diagnostic procedures

Legal Information

FastStart is a trademark of Roche

LightCycler is a registered trademark of Roche

TaqMan is a registered trademark of Roche Molecular Systems, Inc.

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This Item	FSUSGMMRO	FSSGMMRO	FSTMPMMRO
Roche FSUPMMRO FastStart Universal Probe Master (Rox)	Roche FSUSGMMRO FastStart Universal SYBR Green Master (Rox)	Roche FSSGMMRO FastStart SYBR Green Master	Roche FSTMPMMRO FastStart TaqMan^® Probe Master
description sufficient for 250 reactions, sufficient for 1250 reactions, sufficient for 5000 reactions, suitable for qPCR, suitable for RT-qPCR	description -	description sufficient for 500 reactions, sufficient for 5000 reactions, suitable for qPCR, suitable for RT-qPCR	description sufficient for 100 reactions, sufficient for 500 reactions, sufficient for 2000 reactions, suitable for qPCR, suitable for RT-qPCR
technique(s) whole genome amplification: suitable, qPCR: suitable, RT-qPCR: suitable	technique(s) whole genome amplification: suitable, RT-qPCR: suitable	technique(s) RT-qPCR: suitable, whole genome amplification: suitable	technique(s) qPCR: suitable, RT-qPCR: suitable, whole genome amplification: suitable
input purified DNA	input purified DNA	input purified DNA	input purified DNA
manufacturer/tradename Roche	manufacturer/tradename Roche	manufacturer/tradename Roche	manufacturer/tradename Roche
packaging pkg of 5000 x 20 μL reactions (04914058001), pkg of 1250 x 20 μL reactions (04913957001), pkg of 250 x 20 μL reactions (04913949001)	packaging pkg of 200 x 50 μL reactions (04913850001), pkg of 2000 x 50 μL reactions (04913914001)	packaging pkg of 5000 x 20 μL reactions (04673492001), pkg of 500 x 20 μL reactions (04673484001)	packaging pkg of 2000 x 50 μL reactions (04673433001), pkg of 500 x 50 μL reactions (04673417001), pkg of 100 x 50 μL reactions (04673409001)
feature dNTPs included: no, hotstart	feature dNTPs included: no, hotstart	feature dNTPs included, hotstart	feature dNTPs included: no, hotstart
detection method probe-based	detection method probe-based	detection method probe-based	detection method probe-based

Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

does not flash

flash_point_c

does not flash

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