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FastStart Universal SYBR Green Master (Rox)

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Size/SKU	Availability	Price
4 x 1.25 mL	Available to ship TODAYfromMILWAUKEE	$443.00
10 x 5 mL	Available to ship TODAYfromMILWAUKEE	$2,650.00

About This Item

NACRES:

NA.55

UNSPSC Code:

41106300

$443.00

Available to ship TODAYDetails

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Properties

usage

sufficient for 200 reactions, sufficient for 2000 reactions

feature

dNTPs included: no, hotstart

manufacturer/tradename

Roche

packaging

pkg of 200 x 50 μL reactions (04913850001), pkg of 2000 x 50 μL reactions (04913914001)

technique(s)

RT-qPCR: suitable, qPCR: suitable, whole genome amplification: suitable

input

purified DNA

detection method

probe-based

Description

General description

FastStart^™ Universal SYBR^™ Green Master (Rox) is a ready-to-use hot start reaction mix for qPCR and RT-qPCR on all real-time PCR systems requiring normalization with ROX.

SYBR^™ Green I is a DNA double-strand-specific dye. During each phase of DNA synthesis, the SYBR^™ Green I dye, which is included in the reaction mix, binds to the amplified PCR products. The amplicon can be detected by its fluorescence.

Hot start protocols have been shown to significantly improve the specificity, sensitivity, and yield of PCR. Heat-labile blocking groups on some of the amino acid residues of FastStart^™ Taq DNA Polymerase make the modified enzyme inactive at room temperature (+15 to +25°C). Therefore, there is no elongation during the period when primers can non-specifically bind. FastStart^™ Taq DNA Polymerase is activated by removing the blocking groups at a high temperature (i.e., a pre-incubation step at +95°C).

Application

FastStart^™ Universal SYBR^™ Green Master (Rox) can be used for the amplification and detection of any DNA or cDNA target, including those that are GC- or AT-rich.
Combine this master mix with Transcriptor First Strand cDNA Synthesis Kit (Roche) to achieve excellent results in two-step qRT-PCR.
FastStart^™ Universal SYBR^™ Green Master (Rox) has been used in qRT-PCR and qPCR

Features and Benefits

Improve PCR sensitivity and specificity.

Minimize the formation of nonspecific amplification products.

Avoid over-estimation of qPCR results.

Eliminate nonspecific amplification products and primer-dimers that increase the amount of bound quantified SYBR Green I.

Amplify and detect a broad range of DNA or cDNA targets.

Amplify fragments up to 500 bp long, including those that are GC- or AT-rich.

Save time and effort in qPCR preparation.

Eliminate the need to mix components, titrate MgCl₂, or perform other time-consuming optimization steps.

Prevent false positives resulting from carryover contamination.

Use this dUTP-containing mix with Uracil-DNA Glycosylase to eliminate contaminating DNA carried over from previous PCR reactions.

Analysis Note

Function test: Each lot is tested for performance in qPCR using three templates: a GC-rich template, an AT-rich template, and a long template (approximately 440 bp).

Other Notes

FastStart Universal SYBR Green Master (Rox), 2x concentrated master mix that contains FastStart Taq DNA Polymerase, Reaction Buffer, Nucleotides (dATP, dCTP, dGTP, dUTP), SYBR Green I, and a reference dye.

For life science research only. Not for use in diagnostic procedures.

Legal Information

SYBR is a trademark of Thermo Fisher Scientific or its subsidiaries

FastStart is a trademark of Roche

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This Item	FSUPMMRO	FSSGMMRO	EAGLETMMRO
Roche FSUSGMMRO FastStart Universal SYBR Green Master (Rox)	Roche FSUPMMRO FastStart Universal Probe Master (Rox)	Roche FSSGMMRO FastStart SYBR Green Master	Roche EAGLETMMRO EagleTaq Universal Master Mix (ROX)
description -	description sufficient for 250 reactions, sufficient for 1250 reactions, sufficient for 5000 reactions, suitable for qPCR, suitable for RT-qPCR	description sufficient for 500 reactions, sufficient for 5000 reactions, suitable for qPCR, suitable for RT-qPCR	description sufficient for 100 reactions, sufficient for 500 reactions, sufficient for 5000 reactions, suitable for qPCR, suitable for RT-qPCR
technique(s) qPCR: suitable, whole genome amplification: suitable, RT-qPCR: suitable	technique(s) whole genome amplification: suitable, qPCR: suitable, RT-qPCR: suitable	technique(s) qPCR: suitable, whole genome amplification: suitable, RT-qPCR: suitable	technique(s) RT-qPCR: suitable, whole genome amplification: suitable
usage sufficient for 200 reactions, sufficient for 2000 reactions	usage sufficient for 1250 reactions, sufficient for 5000 reactions, sufficient for 250 reactions	usage sufficient for 500 reactions, sufficient for 5000 reactions	usage sufficient for 500 reactions, sufficient for 100 reactions, sufficient for 5000 reactions
detection method probe-based	detection method probe-based	detection method probe-based	detection method probe-based
input purified DNA	input purified DNA	input purified DNA	input purified DNA
packaging pkg of 200 x 50 μL reactions (04913850001), pkg of 2000 x 50 μL reactions (04913914001)	packaging pkg of 1250 x 20 μL reactions (04913957001), pkg of 5000 x 20 μL reactions (04914058001), pkg of 250 x 20 μL reactions (04913949001)	packaging pkg of 500 x 20 μL reactions (04673484001), pkg of 5000 x 20 μL reactions (04673492001)	packaging pkg of 100 x 20 μL reactions (07260288190), pkg of 500 x 20 μL reactions (07260296190), pkg of 5000 x 20 μL reactions (07249926190)
feature hotstart, dNTPs included: no	feature dNTPs included: no, hotstart	feature hotstart, dNTPs included	feature dNTPs included, hotstart

Storage Class

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point (°F)

does not flash

Flash Point (°C)

does not flash

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