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03777

Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid

BioUltra, ≥99.0% (T)

Synonym(s):

EGTA, Egtazic acid, Ethylene-bis(oxyethylenenitrilo)tetraacetic acid, Glycol ether diamine tetraacetic acid

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Size/SKUAvailabilityPrice
10 g

Available to ship TODAYfromMILWAUKEE

$234.00
50 g

Available to ship TODAYfromMILWAUKEE

$705.00

About This Item

Linear Formula:
[-CH2OCH2CH2N(CH2CO2H)2]2
CAS Number:
Molecular Weight:
380.35
UNSPSC Code:
12352106
NACRES:
NA.25
PubChem Substance ID:
EC Number:
200-651-2
Beilstein/REAXYS Number:
1717370
MDL number:

$234.00


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grade

Molecular Biology

Quality Segment

product line

BioUltra

assay

≥99.0% (T)

form

powder

reaction suitability

reagent type: chelator

impurities

DNases, none detected, RNases, none detected, insoluble matter, passes filter test, phosphatases, none detected, proteases, none detected

ign. residue

≤0.05% (as SO4)

loss

≤1.0% loss on drying, 110 °C

mp

241 °C (dec.) (lit.)

solubility

0.1 M NaOH: 0.1 M at 20 °C, clear, colorless

anion traces

sulfate (SO42-): ≤50 mg/kg

cation traces

Al: ≤5 mg/kg, As: ≤0.1 mg/kg, Ba: ≤5 mg/kg, Bi: ≤5 mg/kg, Ca: ≤10 mg/kg, Cd: ≤5 mg/kg, Co: ≤5 mg/kg, Cr: ≤5 mg/kg, Cu: ≤5 mg/kg, Fe: ≤5 mg/kg, K: ≤50 mg/kg, Li: ≤5 mg/kg, Mg: ≤5 mg/kg, Mn: ≤5 mg/kg, Mo: ≤5 mg/kg, Na: ≤200 mg/kg, Ni: ≤5 mg/kg, Pb: ≤5 mg/kg, Sr: ≤5 mg/kg, Zn: ≤5 mg/kg

absorption

cut-off at 263 nm in 1 M NaOH at 0.1 M

SMILES string

OC(=O)CN(CCOCCOCCN(CC(O)=O)CC(O)=O)CC(O)=O

InChI

1S/C14H24N2O10/c17-11(18)7-15(8-12(19)20)1-3-25-5-6-26-4-2-16(9-13(21)22)10-14(23)24/h1-10H2,(H,17,18)(H,19,20)(H,21,22)(H,23,24)

InChI key

DEFVIWRASFVYLL-UHFFFAOYSA-N

Application

A chelating agent useful for the determination of calcium in the presence of magnesium.
Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid can be used to study molecular biology, biochemicals, reagents and chelators. Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid has been used in a study that illustrated the response of the β-cyclodextrin-hemin complex in the induction of cucumber adventitious rooting through heme oxygenase-1-dependent mechanism and calcium signaling.

Biochem/physiol Actions

Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid A is a chelating agent used for the determination of calcium in the presence of magnesium.

Other Notes

Buffer component in dideoxy sequencing of RNA using reverse transcriptase.

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This Item
E0396E3889E8145
description

BioUltra, ≥99.0% (T)

description

BioXtra, ≥97 .0%

description

Molecular Biology, ≥97.0%

description

≥97%

grade

Molecular Biology

grade

-

grade

Molecular Biology

grade

-

assay

≥99.0% (T)

assay

≥97 .0%

assay

≥97.0%

assay

≥97%

product line

BioUltra

product line

BioXtra

product line

-

product line

-

Quality Level

300

Quality Level

400

Quality Level

400

Quality Level

200

anion traces

sulfate (SO42-): ≤50 mg/kg

anion traces

sulfate (SO42-): ≤0.05%

anion traces

-

anion traces

-

impurities

DNases, none detected, insoluble matter, passes filter test, phosphatases, none detected, proteases, none detected, RNases, none detected

impurities

≤0.1% Insoluble matter, ≤0.0005% Phosphorus (P)

impurities

-

impurities

-


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Storage Class

11 - Combustible Solids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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Questions

1–2 of 2 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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