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318337

Acridine Orange hydrochloride hydrate

≥98% (HPLC)

Synonym(s):

3,6-Bis(dimethylamino)acridine hydrochloride

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250 mg

Estimated to ship TODAYfromMILWAUKEE

$62.20
1 g

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$185.00
5 g

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$794.00

About This Item

Empirical Formula (Hill Notation):
C17H19N3 · HCl · xH2O
CAS Number:
65-61-2
Molecular Weight:
301.81 (anhydrous basis)
EC Number:
200-614-0
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
24859123
MDL number:
MFCD00150070
Colour Index Number:
46005
Beilstein/REAXYS Number:
3734978

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Quality Segment

assay

≥98% (HPLC)

form

powder

technique(s)

microbe id | staining: suitable

color

orange

mp

284-287 °C (lit.)

solubility

H2O: 0.1%, clear, orange to red

λmax

492 nm

ε (extinction coefficient)

≥14,000 at 227-233 nm in water at 0.005 g/L, ≥35000 at 265-271 nm in water at 0.005 g/L

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

Cl[H].[H]O[H].CN(C)c1ccc2cc3ccc(cc3nc2c1)N(C)C

InChI

1S/C17H19N3.ClH.H2O/c1-19(2)14-7-5-12-9-13-6-8-15(20(3)4)11-17(13)18-16(12)10-14;;/h5-11H,1-4H3;1H;1H2

InChI key

JUAAUEXLEKAEQC-UHFFFAOYSA-N

Application

Acridine Orange hydrochloride hydrate dye has been used:
  • to stain intracellular acidic vesicles.
  • to measure lysosomal membrane permeability (LMP).
  • or autophagy detection.
A rigorously purified zinc-free material suitable for use in flow cytometry.
Fluorescent stain for nucleic acids in agarose and polyacrylamide gels. Has been used to study apoptosis in various cell culture and tissue models.

Biochem/physiol Actions

Acridine Orange is a metachromatic dye which can stain DNA, RNA and acid glycosaminoglycans. At low concentrations, it intercalates into DNA and precipitates RNA. However, at high concentrations it denatures and precipitates both RNA and DNA. Acridine Orange is also used to analyze autophagy. It enters into acidic organelles in a pH-dependent manner. At neutral pH, acridine orange gives a green fluorescence and in acidic conditions, it accumulates in acidic organelles giving a bright red fluorescence.

Other Notes

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

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This Item
235474390062A8097
Acridine Orange hydrochloride hydrate ≥98% (HPLC)

Sigma-Aldrich

318337

Acridine Orange hydrochloride hydrate

Acridine Orange base Dye content 75 %

Sigma-Aldrich

235474

Acridine Orange base

Thiazole Orange Dye content ~90 %

Sigma-Aldrich

390062

Thiazole Orange

Acridine Orange hydrochloride solution 10 mg/mL in H2O, ≥95.0% (HPLC)

Sigma-Aldrich

A8097

Acridine Orange hydrochloride solution

description

≥98% (HPLC)

description

Dye content 75 %

description

Dye content ~90 %

description

10 mg/mL in H2O, ≥95.0% (HPLC)

technique(s)

microbe id | staining: suitable

technique(s)

microbe id | staining: suitable

technique(s)

titration: suitable

technique(s)

microbe id | staining: suitable

color

orange

color

-

color

-

color

orange to red

solubility

H2O: 0.1%, clear, orange to red

solubility

1 M HCl: 1 mg/mL, clear

solubility

-

solubility

-

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

form

powder

form

powder

form

powder

form

solution

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

Pictograms

Health hazard
GHS08

Signal Word

Warning

Hazard Codes

H341

Hazard Classifications

Muta. 2

Storage Class

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, type N95 (US)

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Questions

  1. Is product 318337, Acridine Orange hydrochloride hydrate, used for sperm chromatin structure assay (SCSA) and sperm DNA structure assay?

    1 answer

    1. Product 318337 is not function tested during quality control testing for use in either the sperm chromatin structure assay (SCSA) or the sperm DNA structure assay. The product is sold for Research Use Only. Quality Control testing includes testing for appearance, infrared spectrum, extinction coefficients at 3 different wavelengths, carbon, nitrogen, purity by HPLC, zinc, solubility, and water content. The literature shows that Acridine Orange is used for differential staining of DNA and RNA and a wide range of other uses. The website does list various uses for product 318337. The list however is not all inclusive of the uses for which the dye can be used. It is up to the customer to determine if the dye is acceptable for their intended use(s).

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