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318604

Ammonium chloride-ammonium hydroxide buffer solution

in H2O

Synonym(s):

Ammonium Buffer Solution

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About This Item

NACRES:
NA.21
PubChem Substance ID:
UNSPSC Code:
12161705
MDL number:
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form

liquid

Quality Segment

composition

NH4Cl, 1 wt. % , NH4OH, 4 wt. % (pH 10 to 11)

concentration

in H2O

pH

10-11

density

0.99 g/mL at 25 °C

SMILES string

N.N.O.Cl

InChI

1S/ClH.2H3N.H2O/h1H;2*1H3;1H2

InChI key

XEXFVRMLYUDDJY-UHFFFAOYSA-N

Application

Ammonium chloride-ammonium hydroxide buffer solution may be used to crystallize proteins.

Biochem/physiol Actions

Ammonium chloride is the salt component of ammonium chloride-ammonium hydroxide buffer system that completely inhibits the BVDV infection of MDBK cells when present at a concentration of 50 mM . Viruses treated with ammonium chloride (NH4Cl) results in loss of infectivity because it neutralizes the pH of endosome. Hence, viruses with low pH-dependent fusion activity became incapable to fuse their envelope with endosomal membrane.

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This Item
T74089036018597
description

in H2O

description

1.0 M, pH 8.5±0.1

description

volatile buffer, ~1.0 M in H2O

description

1 M, suitable for HPLC, LiChropur™

concentration

in H2O

concentration

1.0 M

concentration

~1.0 M in H2O

concentration

1 M

Quality Level

100

Quality Level

200

Quality Level

200

Quality Level

100

form

liquid

form

liquid

form

liquid

form

liquid

pH

10-11

pH

8.5±0.1

pH

8.4-8.6 (25 °C)

pH

8.4-8.6 (20 °C in neat)

density

0.99 g/mL at 25 °C

density

1.02 g/mL at 25 °C (lit.)

density

1.018 g/mL at 20 °C

density

1.015-1.024

composition

NH4Cl, 1 wt. % , NH4OH, 4 wt. % (pH 10 to 11)

composition

-

composition

-

composition

-


Pictograms

CorrosionEnvironment

Signal Word

Danger

Hazard Codes

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Skin Irrit. 2

Storage Class

12 - Non Combustible Liquids

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable



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Questions

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  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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