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61767

Lectin from Triticum vulgaris

lyophilized, ≥55% (GE), powder

Synonym(s):

WGA, Wheat germ agglutinin

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5 mg
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$124.00

About This Item

UNSPSC Code:
12352202
NACRES:
NA.56
MDL number:
Form:
powder
Biological source:
Triticum vulgaris

$124.00


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biological source

Triticum vulgaris

form

powder

quality

lyophilized

concentration

≥55% (GE)

technique(s)

agglutination assay: suitable using fresh human erythrocytes

storage temp.

2-8°C

General description

WGA is not blood group specific but has an affinity for N-acetyl-β-D-glucosaminyl residues and N-acetyl-β-D-glucosamine oligomers. WGA contains no protein-bound carbohydrate.
Lectins are carbohydrate-binding proteins, omnipresent, found in fungi, plants and animals. The structure of lectin is diversely studied in plants and animals. The secondary structure of this protein is rich in β-strands and posses a carbohydrate binding sites on the surface.

Application

Wheat germ agglutinin conjugated to the outside surface of PLGA nanoparticles loaded with paclitaxel was used to target cancer cells that overexpress WGA-binding glycoproteins (Caco-2 and HT-29 cell lines). WGA-NP showed 50-60% greater endocytosis and intracellular retention of paclitaxel than unlabeled nanoparticles.
Lectin from Triticum vulgaris has been used in bone resorption assays.

Biochem/physiol Actions

Lectin is known to be useful in glycoconjugate characterizing, imaging and targeting. Its use in a microarray assay, enable efficient glycome profiling. This is because of its specific interaction with oligosaccharides, glycoproteins and glycolipids. In plants and fungi, lectin defends against pathogens/feeders. Lectin participates in host recognition and tissue adhesion, thereby aids in the pathogenesis of microorganism.

Other Notes

Molecular characteristics and specificity for sugar binding

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description

lyophilized, ≥55% (GE), powder

description

BioReagent, suitable for cell culture, lyophilized powder

description

peroxidase conjugate, lyophilized powder

description

lyophilized powder

technique(s)

agglutination assay: suitable using fresh human erythrocytes

technique(s)

cell culture | mammalian: suitable

technique(s)

-

technique(s)

-

biological source

Triticum vulgaris

biological source

-

biological source

Triticum vulgaris

biological source

-

concentration

≥55% (GE)

concentration

-

concentration

-

concentration

-

form

powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

quality

lyophilized

quality

-

quality

-

quality

-


Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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