Activation is not required for this preparation. However, the beads should be washed thoroughly prior to use y with 5-10 column volumes of buffer, to remove the sodium azide in the storage buffer. Please see the link below to review the datasheet for product S1638, this may be applied to this material.
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/424/273/s1638pis-ms.pdf
85881
Streptavidin, immobilized on Agarose CL-4B
suitable for affinity chromatography
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| Size/SKU | Availability | Price |
|---|---|---|
1 mL | Available to ship TODAYfromMILWAUKEE | $227.00 |
About This Item
NACRES:
NA.21
UNSPSC Code:
12190000
form
suspension
Quality Segment
contains
0.02% NaN3 as preservative
technique(s)
affinity chromatography: suitable
capacity
≥15 μg/mL, packed gel capacity (biotin)
storage temp.
2-8°C
General description
streptavidin attached to 4% beaded cross-linked agarose via a C6-spacer (1.2 mg streptavidin/ml packed gel)
Physical form
50% suspension in 0.01 M sodium phosphate buffer, pH 7.2, containing 0.15 M sodium chloride
1 of 1
This Item | |||
|---|---|---|---|
| description suitable for affinity chromatography | description Column or batch isolation of biotinylated molecules | description 5 mg Streptavidin for ELISA & Western Blotting. | description buffered aqueous suspension |
| contains 0.02% NaN3 as preservative | contains - | contains - | contains - |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. - | storage temp. 2-8°C |
| form suspension | form liquid | form - | form buffered aqueous suspension |
| technique(s) affinity chromatography: suitable | technique(s) - | technique(s) ELISA: suitable, western blot: suitable | technique(s) affinity chromatography: suitable |
| capacity ≥15 μg/mL, packed gel capacity (biotin) | capacity - | capacity - | capacity ≥15 μg/mL binding capacity (biotin) |
| Quality Level 100 | Quality Level 100 | Quality Level 100 | Quality Level 200 |
Storage Class
10 - Combustible liquids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
PPE (personal protective equipment)
Eyeshields, Gloves
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I want to know if before using the beads I need activate it with any specific solution. Could you help me please.
1 answer-
Helpful?
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