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C3023

CHAPS hydrate

≥98% (HPLC)

Promotion Available

Synonym(s):

3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate hydrate, CHAPS

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1 g

Available to ship TODAYfromMILWAUKEE

$70.00
$59.50
5 g

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$210.00
1 kit

Estimated to ship onSeptember 14, 2026fromMILWAUKEE

$735.00
25 g

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$846.00
100 g

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$2,300.00

About This Item

Empirical Formula (Hill Notation):
C32H58N2O7S · xH2O
CAS Number:
Molecular Weight:
614.88 (anhydrous basis)
NACRES:
NA.21
PubChem Substance ID:
UNSPSC Code:
12161900
MDL number:

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biological source

bovine bile

Quality Segment

grade

HPLC grade

description

zwitterionic

assay

≥98% (HPLC)

form

powder

mol wt

614.88 g/mol (anhydrous basis)

storage condition

dry at room temperature (tightly closed)

technique(s)

dialysis: suitable, electrophoresis: suitable

impurities

<0.04% DMF

CMC

8 mM

mp

157 °C (dec.) (lit.)

transition temp

cloud point >100 °C

solubility

water: 50 mg/mL

application(s)

advanced drug delivery

shipped in

ambient

storage temp.

2-30°C

SMILES string

O.C[C@H](CCC(=O)NCCC[N+](C)(C)CCCS([O-])(=O)=O)[C@H]1CC[C@H]2[C@@H]3[C@H](O)C[C@@H]4C[C@H](O)CC[C@]4(C)[C@H]3C[C@H](O)[C@]12C

InChI

1S/C32H58N2O7S.H2O/c1-21(8-11-29(38)33-14-6-15-34(4,5)16-7-17-42(39,40)41)24-9-10-25-30-26(20-28(37)32(24,25)3)31(2)13-12-23(35)18-22(31)19-27(30)36;/h21-28,30,35-37H,6-20H2,1-5H3,(H-,33,38,39,40,41);1H2/t21-,22+,23-,24-,25+,26+,27-,28+,30+,31+,32-;/m1./s1

InChI key

SJCUTFKCLFLIFE-JWTJKVBLSA-N

General description

CHAPS hydrate, a zwitterionic detergent derived from Cholic acid, stands out as an excellent tool in biochemical and cell biology research, particularly in the solubilization of membrane proteins. This versatile detergent, combining features of sulfobetaine-type and bile-acid detergents, exhibits a remarkable solubility rate of over 50% in aqueous solutions. It serves a pivotal role in stabilizing protein-DNA complexes, ensuring the retention of biochemical activity in solution.

Widely utilized as a buffer in various research applications, CHAPS hydrate acts as an amphiphilic surfactant, proving effective in solubilizing plasma membrane proteins and diluting assay components. Notably, its easy removal through dialysis or gel filtration adds to its appeal, making it a preferred choice for molecular mass determination by fluorometry. This feature underscores CHAPS hydrate′s adaptability in diverse experimental setups. However, caution is advised in assays involving polyvalent metals or metal ions sensitive to nonspecific chelating agents. Beyond its conventional use, CHAPS hydrate finds application as a polymer matrix in binding assays, effectively removing metal ions from protein solutions.

Application

0.2 w/v of CHAPS has been used to remove cerebral endothelium by brain perfusion. 2% (w/v) CHAPS has been used as rehydration buffer along with Thiourea and Urea in protein extraction. 10 mM CHAPS has been used along with 0.15 M NaCl, Tris–Cl, pH 7.6, EDTA as lysis buffer in Western Blot analysis. CHAPS has been used as hybridization buffer in in-situ hybridization method of immature mammalian CNS.

Features and Benefits

  • Suitable for Biochemical and Cell Biology research
  • Can be used in Dialysis and Electrophoresis
  • High purity product for research applications

Other Notes

For additional information on our range of Biochemicals, please complete this form.

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assay

≥98% (HPLC)

assay

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assay

≥98.0% (TLC)

form

powder

form

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form

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form

powder

grade

HPLC grade

grade

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grade

Molecular Biology

grade

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technique(s)

electrophoresis: suitable, dialysis: suitable

technique(s)

protein quantification: suitable, electrophoresis: suitable

technique(s)

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technique(s)

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application(s)

advanced drug delivery

application(s)

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application(s)

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application(s)

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storage temp.

2-30°C

storage temp.

room temp

storage temp.

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storage temp.

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pictograms

Exclamation mark

signalword

Warning

hcodes

Hazard Classifications

Acute Tox. 4 Oral

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable



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Questions

1–2 of 2 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

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