This product is Collagenase Type IV, which digests type IV collagen, a major structural component of the basement membrane. It is likely that this product will affect the extracellular matrix to some extent. This product has not been tested for this specific application. Reviewing literature on this topic may provide additional insights.
C4-BIOC
Collagenase Type IV, Cls IV
Synonym(s):
Collagen A, Type IV Gelatinase
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| Size/SKU | Availability | Price |
|---|---|---|
100 mg | Available to ship TODAYfromMILWAUKEE | $62.10 |
1 g | Available to ship TODAYfromMILWAUKEE | $403.00 |
About This Item
NACRES:
NA.78
UNSPSC Code:
12352204
General description
Collagenase Type IV, Cls IV is a member of the mammalian extracellular neutral metalloproteinases family. It digests type IV collagen, which is a major structural constituent of the basement membrane.
This product is identical to Biochrom GmbH part numbers C4-22 and C4-28. Enzyme blending from collagenase, clostripain, with tryptic and proteolytic activities. Recommended for the isolation of Islets of Langerhans. Specific activity is 125 to 250 Mandl units per milligram of powdered substance.
Application
Collagenase Type IV, Cls IV may be used:
- as a component in Dulbecco′s modified Eagle′s medium (DMEM)/F12 to digest endometrial samples isolated from in vitro fertilization (IVF) patients
- for collagenase perfusion to isolate hepatocytes using collagenase-liver perfusion method in rats
- in the isolation of rat glomerular endothelial cells (GEndCs)
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Does it detach the collagen IV without damaging the ECM or affecting the structure of it?
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What is the storage condition and shelf life after reconstituting the product?
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The long-term stability of this product in solution has not been determined. However, a similar Collagenase type IV product, C5138, notes that solutions are stable for several months when stored in aliquots at -20°C. Please see the link below to review the datasheet of a similar item:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/380/876/c5138pis.pdfHelpful?
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How does one solubilize collagenase, and what is the usage concentration for digestion and cell isolation?
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The substance is soluble at 50mg/ml and can be dissolved in water, although it is recommended to use saline, NaCl, DPBS, Hanks, or a culture medium. It is worth noting that in peer-reviewed papers at sial.com, glomerular endothelial cells were isolated at 0.1% in DMEM.
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