This product has not been tested for use as a cell lysis agent. M-Clarity Quality Level 500 is necessary for pharmaceutical and regulated applications. Product S7705 is a cell lysis buffer, but its Quality Level is 100.
C5070
CHAPS hydrate
BioXtra, ≥98% (TLC)
Synonym(s):
3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate hydrate, CHAPS
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| Size/SKU | Availability | Price |
|---|---|---|
1 g | Check Cart for Availability | $134.00 |
5 g | Available to ship TODAYfromMILWAUKEE | $453.00 |
About This Item
Empirical Formula (Hill Notation):
C32H58N2O7S · xH2O
CAS Number:
Molecular Weight:
614.88 (anhydrous basis)
NACRES:
NA.21
PubChem Substance ID:
UNSPSC Code:
12161900
MDL number:
description
zwitterionic
Quality Segment
product line
BioXtra
assay
≥98% (TLC)
form
powder
mol wt
614.88 g/mol (anhydrous basis)
technique(s)
protein purification: suitable, protein quantification: suitable
impurities
≤0.001% Phosphorus (P), ≤0.1% Insoluble matter, <0.04% DMF
ign. residue
≤0.1%
mp
157 °C (dec.) (lit.)
solubility
H2O: 0.1 M, clear, colorless
anion traces
chloride (Cl-): ≤0.05%
cation traces
Al: ≤0.0005%, Ca: ≤0.0005%, Cu: ≤0.0005%, Fe: ≤0.0005%, K: ≤0.005%, Mg: ≤0.0005%, NH4+: ≤0.05%, Na: ≤0.005%, Pb: ≤0.001%, Zn: ≤0.0005%
SMILES string
O.C[C@H](CCC(=O)NCCC[N+](C)(C)CCCS([O-])(=O)=O)[C@H]1CC[C@H]2[C@@H]3[C@H](O)C[C@@H]4C[C@H](O)CC[C@]4(C)[C@H]3C[C@H](O)[C@]12C
InChI
1S/C32H58N2O7S.H2O/c1-21(8-11-29(38)33-14-6-15-34(4,5)16-7-17-42(39,40)41)24-9-10-25-30-26(20-28(37)32(24,25)3)31(2)13-12-23(35)18-22(31)19-27(30)36;/h21-28,30,35-37H,6-20H2,1-5H3,(H-,33,38,39,40,41);1H2/t21-,22+,23-,24-,25+,26+,27-,28+,30+,31+,32-;/m1./s1
InChI key
SJCUTFKCLFLIFE-JWTJKVBLSA-N
General description
CHAPS is a non-denaturing, zwitterionic sulfobetaine detergent. It is mild and solubilizes membrane proteins and receptors. It is useful for breaking protein-protein interactions. It is a derivative of the naturally occurring bile salts. It has small micellar molecular weight (6,150) and high critical micellar concentration (6 - 10 mM) allowing it to be removed from samples by dialysis.
Application
- 10mM CHAPS detergent has been used in the process of ion-exchange chromatography for improved purification.
- 0.5 w/v of CHAPS is a component of high salt buffer in protein purification alongside HEPES, NaCl and EDTA.
- HEPES buffer has been used as lysis buffer in immunoprecipitation, supplemented with 1% CHAPS.
1 of 1
This Item | |||
|---|---|---|---|
| description BioXtra, ≥98% (TLC) | description ≥98% (HPLC) | description BioReagent, suitable for electrophoresis, ≥98% (HPLC) | description 98% (from N), suitable for dialysis, solubility: >50% (Aqueous solutions) |
| technique(s) protein purification: suitable, protein quantification: suitable | technique(s) dialysis: suitable, electrophoresis: suitable | technique(s) electrophoresis: suitable, protein quantification: suitable | technique(s) dialysis: suitable |
| assay ≥98% (TLC) | assay ≥98% (HPLC) | assay ≥98% (HPLC) | assay 98% (from N) |
| form powder | form powder | form powder | form crystals |
| impurities ≤0.001% Phosphorus (P), <0.04% DMF, ≤0.1% Insoluble matter | impurities <0.04% DMF | impurities <0.04% DMF | impurities - |
| mol wt 614.88 g/mol (anhydrous basis) | mol wt 614.88 g/mol (anhydrous basis) | mol wt 614.88 g/mol (anhydrous basis) | mol wt micellar avg mol wt 6150, 614.88 g/mol (anhydrous basis) |
| Quality Level 400 | Quality Level 400 | Quality Level 200 | Quality Level - |
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signalword
Warning
hcodes
Hazard Classifications
Acute Tox. 4 Oral
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Hi, Could CHAPS C5070 quality level 400 be used as a cell lysis agent at the harvest stage for a human biological therapeutic?
1 answer-
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