09042001
CACO-2
human cervix, Epithelial
Synonym(s):
Caco-II Cells, Caco2 Cells
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About This Item
UNSPSC Code:
41106514
Biological source:
human colon
Relevant disease(s):
cancer
Growth mode:
(Adherent)
Karyotype:
(Hypertetraploid, modal no. 96)
Morphology:
Epithelial
Product Name
CACO-2, Caucasian colon adenocarcinoma
biological source
human colon
Quality Level
form
liquid
growth mode
(Adherent)
karyotype
(Hypertetraploid, modal no. 96)
morphology
Epithelial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
CACO-2 has been used in the cytotoxicity studies with high pressure-assisted extraction (HPE) fraction of nettle leaf extracts.[1]
CACO-2 may be used as a cell model to study:
CACO-2 may be used as a cell model to study:
- in vitro permeability assay with various biopharmaceuticals
- in iron uptake and absorption studies
- in the cytotoxicity assay with surfactants and transporter inhibitors
- in liposome formulated drug uptake and cytotoxicity studies
Biochem/physiol Actions
Human caucasian colon adenocarcinoma, intestinal permeability characteristics tested
Isolated from a primary colonic tumour in a 72-year-old Caucasian male using the explant culture technique. Forms moderately well differentiated adenocarcinomas consistent with colonic primary grade II, in nude mice. This CACO-2 catalogue number has undergone testing for intestinal permeability: Dome, microvilli and tight junction formation and transport functionality tested and confirmed present at ECACC.
STR-PCR Data:
Amelogenin: X
CSF1PO: 11
D13S317: 11,13,14
D16S539: 12,13
D5S818: 12,13
D7S820: 11,12
THO1: 6
TPOX: 9,11
vWA: 16,18
Amelogenin: X
CSF1PO: 11
D13S317: 11,13,14
D16S539: 12,13
D5S818: 12,13
D7S820: 11,12
THO1: 6
TPOX: 9,11
vWA: 16,18
Caco-2 (Cancer coli-2) is derived from colorectal adenocarcinoma and grows as an adherent monolayer of epithelial cells. Caco-2 cell line is a widely used intestinal epithelium model and has unique spontaneous differentiation and confluence property. It actively transports vitamins, hormones, amino acids and sugars. Caco-2 expresses various enzymes, ionic and non-ionic transporters similar to normal human epithelia. Caco-2 cell line models grow on filter support. They have simplicity and reproducibility and provide reliable information. Since Caco-2 mimics the intestinal epithelium, it is the preferred cell model for cytotoxic studies,[1] food and drug transport studies.
Preparation Note
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum FBS / FCS.
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. NB: During routine subculture the cells should always be subcultured before they achieve confluence. Cells may show the appearance of circular vacuoles in the cytoplasm. These may increase in frequency as the culture density increases to confluence. To reduce their frequency, media change confluent cultures after 2-3 days if not subcultured. Cells can clump if not separated into a single cell suspension when split.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
NaviCyte Scientific holds the exclusive commercial distribution rights to the CACO-2 cell line deposited by the Memorial Sloan-Kettering Cancer Center. Note: All uses of Catalogue Numbers 86010202 and 09042001, other than for research by a non-commercial or academic entity, require a license and use authorization from NaviCyte Scientific under its exclusive arrangement with Memorial Sloan-Kettering Cancer Center. For information on the licensing terms, please contact NaviCyte Scientific via [email protected] or (+1) 973-868-6100.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to [email protected].
Disclaimer
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
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This Item | |||
|---|---|---|---|
| karyotype (Hypertetraploid, modal no. 96) | karyotype Dog | karyotype - | karyotype - |
| growth mode (Adherent) | growth mode Adherent | growth mode N/A | growth mode - |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) - |
| receptors Not specified | receptors Not specified | receptors - | receptors - |
| biological source human colon | biological source canine kidney | biological source - | biological source - |
| relevant disease(s) cancer | relevant disease(s) - | relevant disease(s) - | relevant disease(s) - |
Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
188.6 °F - closed cup
flash_point_c
87 °C - closed cup
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Comparison study of oral iron preparations using a human intestinal model
Zariwala MG, et al.
Scientia Pharmaceutica, 81, 1123-1140 (2013)
Caco-2 Cell Line
Verhoeckx K, et al.
The Impact of Food Bioactives on Health, 103-111 (2015)
High-throughput screening of excipients with a biological effect: a kinetic study on the effects of surfactants on efflux-mediated transport
Pollard J, et al.
The Journal of Pharmacy and Pharmacology, 71, 889-897 (2019)
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 09042001-1VL | 04061838653055 |