This product has not been tested for use in any specific application. The material is soluble in DMSO, Dimethylformamide, and water. Please see the link below to review additional publications that may be helpful:
https://www.sciencedirect.com/topics/chemistry/ethidium-homodimer
46043
Ethidium homodimer
suitable for fluorescence, ~90% (HPCE)
Synonym(s):
EtDi, EthD-1
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| Size/SKU | Availability | Price |
|---|---|---|
1 mg | Available to ship TODAYfromMILWAUKEE | $452.00 |
5 mg | Check Cart for Availability | $1,830.00 |
About This Item
Empirical Formula (Hill Notation):
C46H50Cl4N8
CAS Number:
Molecular Weight:
856.75
NACRES:
NA.32
PubChem Substance ID:
UNSPSC Code:
12161505
EC Number:
263-325-9
MDL number:
Quality Segment
description
suitable for the fluorogenic detection of DNA
assay
~90% (HPCE)
form
solid
mol wt
856.75 g/mol
concentration
≤100% (5,5′-[Ethylenebis(iminotrimethylene)]bis[3,8-diamino-6-phenylphenanthridinium] dichloride dihydrochloride)
technique(s)
protein detection: suitable
color
red to dark red
mp
≥250 °C (lit.)
solubility
DMF: soluble, DMSO: soluble, H2O: soluble
fluorescence
λex 493 nm in H2O, λex 528 nm; λem 617 nm in TE buffer; DNA
suitability
suitable for fluorescence
storage temp.
−20°C
SMILES string
Cl.Cl.[Cl-].[Cl-].Nc1ccc2c(c1)c(-c3ccccc3)[n+](CCCNCCNCCC[n+]4c(-c5ccccc5)c6cc(N)ccc6c7ccc(N)cc47)c8cc(N)ccc28
InChI
1S/C46H46N8.4ClH/c47-33-13-17-37-39-19-15-35(49)29-43(39)53(45(41(37)27-33)31-9-3-1-4-10-31)25-7-21-51-23-24-52-22-8-26-54-44-30-36(50)16-20-40(44)38-18-14-34(48)28-42(38)46(54)32-11-5-2-6-12-32;;;;/h1-6,9-20,27-30,49-52H,7-8,21-26,47-48H2;4*1H
InChI key
ZKAJUKABUUJRLB-UHFFFAOYSA-N
Application
Ethidium homodimer (EtDi, EthD-1) is used as a staining dye for ssDNA, dsDNA, RNA, oligonucleotides, and triplex DNA. EthD-1 does not cross intact/viable cell membranes and can be used to test cell viability.
Staining dye for ssDNA, dsDNA, RNA, oligonucleotides, and triplex DNA. It will not cross intact cell membranes and can be used to test cell viability.
Packaging
Bottomless glass bottle. Contents are inside inserted fused cone.
1 of 1
This Item | |||
|---|---|---|---|
| description suitable for fluorescence, ~90% (HPCE) | description ≥95% (Run on Ethidium Homodimer before dissolution in DMSO, TLC), 2 mM in DMSO | description Selectophore™ | description Selectophore™, ≥99.0% |
| suitability suitable for fluorescence | suitability - | suitability - | suitability - |
| color red to dark red | color red | color - | color - |
| fluorescence λex 528 nm; λem 617 nm in TE buffer; DNA, λex 493 nm in H2O | fluorescence - | fluorescence - | fluorescence - |
| concentration ≤100% (5,5′-[Ethylenebis(iminotrimethylene)]bis[3,8-diamino-6-phenylphenanthridinium] dichloride dihydrochloride) | concentration 2 mM in DMSO | concentration - | concentration - |
| form solid | form liquid | form powder | form - |
| solubility DMF: soluble, H2O: soluble, DMSO: soluble | solubility - | solubility - | solubility - |
Signal Word
Danger
Hazard Codes
Precautionary Statements
Storage Class
4.3 - Hazardous materials which set free flammable gases upon contact with water
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
PPE (personal protective equipment)
dust mask type N95 (US), Eyeshields, Gloves
Hazard Classifications
Carc. 2 - Eye Dam. 1 - Skin Corr. 1B - Water-react. 3
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