The solubility of this product has not been determined. However, various sources state that this material is soluble in DMSO and only sparingly soluble in water. The exact concentrations are not reported.
73144
Resorufin
95%
Synonym(s):
7-Hydroxy-3H-phenoxazin-3-one, NSC 12097
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 20 mg | Please contact Customer Service for Availability | $289.00 |
About This Item
Empirical Formula (Hill Notation):
C12H7NO3
CAS Number:
Molecular Weight:
213.19
UNSPSC Code:
12171500
NACRES:
NA.32
PubChem Substance ID:
EC Number:
211-241-8
Beilstein/REAXYS Number:
174850
MDL number:
Quality Level
assay
≥95.0% (HPLC), 95%
form
solid
mp
>300 °C (lit.)
storage temp.
−20°C
SMILES string
O=C(C=C1)C=C2C1=NC3=CC=C(O)C=C3O2
InChI
1S/C12H7NO3/c14-7-1-3-9-11(5-7)16-12-6-8(15)2-4-10(12)13-9/h1-6,14H
InChI key
HSSLDCABUXLXKM-UHFFFAOYSA-N
General description
Red fluorophore standard. Widely used in enzyme assay systems. Absorption and fluorescence of resorufin are pH dependent. Below the pKa (~6.0), Abs. shifts to ~ 480 nm and both E and fluorescence quantum yield are markedly lower. Resorufin is unstable in the presence of thiols such as dithiothreitol (DTT) and 2-mercaptoethanol.
Resorufin has high extinction coefficient and good chemical and photostability. These properties of resorufin make it perfect for its usage in inverse fluorescence assay to detect H2O2 by measuring the disappearance of its fluorescence upon horseradish peroxidase-mediated oxidation to a nonfluorescent derivative.
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This Item | |||
|---|---|---|---|
| form solid | form powder | form powder | form powder |
| Quality Level 100 | Quality Level 200 | Quality Level 100 | Quality Level 200 |
| assay ≥95.0% (HPLC), 95% | assay ~95% | assay - | assay ≥90% |
| storage temp. −20°C | storage temp. −20°C | storage temp. room temp | storage temp. −20°C |
| mp >300 °C (lit.) | mp - | mp >300 °C (lit.) | mp - |
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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A stable nonfluorescent derivative of resorufin for the fluorometric determination of trace hydrogen peroxide: applications in detecting the activity of phagocyte NADPH oxidase and other oxidases.
Zhou M, et al.
Analytical Biochemistry, 253(2), 162-168 (1997)
Matteo Miceli et al.
Molecules (Basel, Switzerland), 24(12) (2019-06-19)
Monoacylglycerol lipase (MAGL) is a serine hydrolase that has a key regulatory role in controlling the levels of 2-arachidonoylglycerol (2-AG), the main signaling molecule in the endocannabinoid system. Identification of selective modulators of MAGL enables both to provide new tools
Ahmed A El-Sherbeni et al.
Drug metabolism and disposition: the biological fate of chemicals, 42(9), 1498-1507 (2014-06-28)
Cytochrome P450 (P450) enzymes mediate arachidonic acid (AA) oxidation to several biologically active metabolites. Our aims in this study were to characterize AA metabolism by different recombinant rat P450 enzymes and to identify new targets for modulating P450-AA metabolism in
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 73144-20MG | 04061833646830 |