This product, a mixture of guanidine thiocyanate and phenol in a monophase solution, effectively dissolves DNA, RNA, and protein on homogenization or lysis of tissue sample. After adding chloroform or 1-bromo3-chloropropane and centrifuging, the mixture separates into 3 phases: an aqueous phase containing the RNA, the interphase containing DNA, and an organic phase containing proteins. Each component can then be isolated after separating the phases. Please see the link below to review further information as well as review specific protocols:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/294/543/t9424bulletin.pdf
93289
TRI Reagent®
Synonym(s):
DNA/RNA/protein extraction reagent, DNA/RNA/protein isolation reagent, DNA/RNA/protein purification reagent, single step RNA extraction reagent, total RNA extraction reagent, TRI Reagent® RNA Isolation Reagent
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| Size/SKU | Availability | Price |
|---|---|---|
25 mL | Available to ship TODAYfromMILWAUKEE | $124.00 |
100 mL | Available to ship TODAYfromMILWAUKEE | $378.00 |
About This Item
$124.00
Available to ship TODAYDetails
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form
liquid
Quality Segment
usage
mL sufficient for 107 cells, mL sufficient for 100 mg tissue
technique(s)
whole genome amplification: suitable
impurities
phenol
General description
TRI reagent® also known as TRIzol, is a monophasic solution of phenol and guanidinium isothiocyanate. It has a potential, to simultaneously solubilize biological material and denatures protein. It is widely used for deproteinizing RNA. This improved version of the single-step total RNA isolation reagent developed by Chomczynski allows a quick, economical, and efficient isolation of total RNA from different biological samples. This product is pure (A260/A280 ~1.8) and can be directly used in Northern blotting. Simultaneous isolation of DNA, RNA and proteins is also possible. 1 mL is sufficient for the extraction of 100 mg tissue or 107 cells.
Application
TRI Reagent® has been used for isolating RNA from various types of cells or tissues. It may also be used for processing tissues, cells cultured in monolayer, or cell pellets.
TRI Reagent is an improved version of the single-step total RNA isolation reagent developed by Chomczynski. The RNA isolation method based on this reagent is widely used and proven for RNA applications. It is ideal for quick, economical, and efficient isolation of total RNA or the simultaneous isolation of RNA, DNA, and proteins from samples of human, animal, plant, yeast, bacterial, and viral origin.
Features and Benefits
- Easily scalable RNA isolation
- Works with many sources: human, plant, yeast, bacterial, or viral
- Better yields than traditional guanidine thiocyanate/cesium chloride methods
Legal Information
TRI Reagent is a registered trademark of Molecular Research Center, Inc.
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This Item | |||
|---|---|---|---|
| description - | description For processing tissues, cells cultured in monolayer or cell pellets | description LS, For processing fluid samples such as cell suspensions, CSF, and amniotic fluid. | description For processing total and small RNA from human, animal, plant, bacterial,and viral samples |
| technique(s) whole genome amplification: suitable | technique(s) whole genome amplification: suitable | technique(s) whole genome amplification: suitable | technique(s) whole genome amplification: suitable |
| Quality Level 100 | Quality Level 200 | Quality Level 100 | Quality Level 100 |
| form liquid | form - | form - | form - |
| impurities phenol | impurities - | impurities - | impurities - |
| usage mL sufficient for 100 mg tissue, mL sufficient for 107 cells | usage mL sufficient for 107 cells, mL sufficient for 100 mg tissue (or) | usage 0.75 mL sufficient for 0.25 mL fluid samples | usage mL sufficient for 100 mg tissue (or), mL sufficient for 107 cells |
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Danger
Hazard Classifications
Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Muta. 2 - Skin Corr. 1B - STOT RE 2
target_organs
Nervous system,Kidney,Liver,Skin
supp_hazards
Storage Class
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
wgk
WGK 2
flash_point_f
174.2 °F - closed cup
flash_point_c
79 °C - closed cup
ppe
Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter
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Excuse me, what is the principle of trizol reagent to separate DNA and RNA?
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