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93862

Nitrotetrazolium Blue chloride

90.0-110.0% (calc. on dry substance, T), powder

Synonym(s):

2,2′-bis(4-Nitrophenyl)-5,5′-diphenyl-3,3′-(3,3′-dimethoxy-4,4′-diphenylene)ditetrazolium chloride, 3,3′-(3,3′-Dimethoxy-4,4′-biphenylene)bis[2-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride], p-Nitro-Blue tetrazolium chloride, p-Nitrotetrazolium blue, NBT, Nitro BT

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Size/SKUAvailabilityPrice
100 mg

Available to ship TODAYfromMILWAUKEE

$54.60
500 mg

Available to ship TODAYfromMILWAUKEE

$193.00
2.5 g

Available to ship TODAYfromMILWAUKEE

$760.00

About This Item

Linear Formula:
C40H30N10O6 · 2Cl
CAS Number:
Molecular Weight:
817.64
NACRES:
NA.54
PubChem Substance ID:
UNSPSC Code:
12171500
MDL number:
Beilstein/REAXYS Number:
4115923

$54.60


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Product Name

Nitrotetrazolium Blue chloride, 90.0-110.0% (calc. on dry substance, T)

Quality Segment

assay

90.0-110.0% (calc. on dry substance, T)

form

powder

impurities

≤10% solvent content, ≤10% water

λ

in methanol

UV absorption

λ: 252-262 nm Amax

storage temp.

2-8°C

SMILES string

[Cl-].[Cl-].COc1cc(ccc1-[n+]2nc(nn2-c3ccc(cc3)[N+]([O-])=O)-c4ccccc4)-c5ccc(c(OC)c5)-[n+]6nc(nn6-c7ccc(cc7)[N+]([O-])=O)-c8ccccc8

InChI

1S/C40H30N10O6.2ClH/c1-55-37-25-29(13-23-35(37)47-43-39(27-9-5-3-6-10-27)41-45(47)31-15-19-33(20-16-31)49(51)52)30-14-24-36(38(26-30)56-2)48-44-40(28-11-7-4-8-12-28)42-46(48)32-17-21-34(22-18-32)50(53)54;;/h3-26H,1-2H3;2*1H/q+2;;/p-2

InChI key

FSVCQIDHPKZJSO-UHFFFAOYSA-L

Biochem/physiol Actions

Nitrotetrazolium blue chloride (NBT) is used in staining, visualizing and evaluating the damage in brain sections or heart tissues. The principle of the technique is that the redox enzymes like nicotinamide adenine dinucleotide NAD(H) and cofactors present in the cell convert colorless tetrazolium salt to blue formazan. The dead cells remain unstained. It is used in the dual-staining along with methyl green for the hyperspectral microscopic imaging analysis of colon tissues for the diagnosis of colon diseases. NBT is also used for diagnosing microbial infections and phagocytic defects. In conventional chromogenic methods, NBT/ 5-bromo-4-chloro-3-indolyl phosphate (BCIP) is used as a substrate for alkaline phosphatase enzyme reaction, in which NBT is reduced to insoluble purple diformazan.

Analysis Note

solubility:
250 mg in 5 mL methanol: yellow to very dark yellow and light brown-yellow to very dark brown-yellow

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This Item
N6876484235N6639
description

90.0-110.0% (calc. on dry substance, T)

description

≥90.0% (HPLC)

description

NADPH-diaphorase substrate that competitively inhibits nitric oxide synthase (IC50 = 3-4 µM).

description

powder, electrophoresis grade

assay

90.0-110.0% (calc. on dry substance, T)

assay

≥90.0% (HPLC)

assay

≥98% (TLC)

assay

>90%

Quality Level

100

Quality Level

200

Quality Level

100

Quality Level

100

form

powder

form

powder

form

crystalline solid

form

powder

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

UV absorption

λ: 252-262 nm Amax

UV absorption

-

UV absorption

-

UV absorption

-

impurities

≤10% solvent content, ≤10% water

impurities

≤10% solvent

impurities

-

impurities

-


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Warning

Hazard Classifications

Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable



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