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F7425

ANTI-FLAG®

affinity isolated antibody, buffered aqueous solution

ANTI-FLAG® antibody produced in rabbit

Sinonimo/i:

Anti-ddddk, Anti-dykddddk

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A voi/SKUDisponibilitàPrezzo
0.2 mg
Per informazioni sulla disponibilità, contatta il Servizio Clienti.
603,00 €

Informazioni su questo articolo

UNSPSC Code:
12352203
NACRES:
NA.32
MDL number:
MFCD02262912
Conjugate:
unconjugated
Clone:
polyclonal
Application:
DB, IF, IP, WB CL
Citations:
3023

603,00 €

Per informazioni sulla disponibilità, contatta il Servizio Clienti.
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biological source

rabbit

Quality Segment

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

all

concentration

~0.8 mg/mL

technique(s)

dot blot: 1-2.5 μg/mL, immunoprecipitation (IP): 4-8 μg using amino terminal FLAG-BAP fusion protein from E. coli crude lysate, indirect immunofluorescence: 5-10 μg/mL using 293T cells transfected with a plasmid encoding FLAG-JNK, western blot (chemiluminescent): 1-2.5 μg/mL using an E. coli periplasmic extract expressing an N-terminal FLAG fusion protein

isotype

IgG

immunogen sequence

DYKDDDDK

shipped in

dry ice

storage temp.

−20°C

General description

ANTI-FLAG, l′anticorpo policlonale per affinità anti-FLAG di coniglio riconosce l′epitopo FLAG posto sulle proteine di fusione FLAG. Questo anticorpo reagisce con le proteine di fusione FLAG N-terminali, N-terminali-met e C-terminali.
L′epitopo FLAG è una proteina costituita da otto aminoacidi con un sito di scissione per l′enterochinasi.

Immunogen

FLAG; sequenza peptidica DYKDDDDK

Application

L′anticorpo riconosce l′epitopo FLAG sulle proteine di fusione taggate con FLAG in posizione N-terminale o C-terminale applicando i saggi dot blot, immunoblot, di immunoprecipitazione e immunocitochimici.
Per scoprire tutti i dettagli sul prodotto è possibile consultare il nostro portale suitag FLAG®.

Biochem/physiol Actions

L′epitopo FLAG ha un ruolo cruciale nella purificazione per immunoaffinità delle proteine di fusione.

Physical form

Soluzione in tampone fosfato salino 0,01 M, pH 7,4, contenente l′1% di albumina da siero bovino e 15 mM di sodio azide.

Preparation Note

Purificato con cromatografia di affinità su una colonna contenente il peptide immunizzante.

Other Notes

Vincitore del CiteAb Award 2024 per i fornitori che hanno conseguito risultati nella ricerca sul Parkinson

Legal Information

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

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Questo articolo
SAB4301135F2555F1804
ANTI-FLAG® affinity isolated antibody, buffered aqueous solution

Millipore

F7425

ANTI-FLAG®

Anti-FLAG Tag antibody produced in rabbit affinity isolated antibody

Sigma-Aldrich

SAB4301135

Anti-FLAG Tag antibody produced in rabbit

Rabbit Monoclonal ANTI-FLAG® Clone SIGI-25 clone SIG1-25, ascites fluid

Sigma-Aldrich

F2555

Rabbit Monoclonal ANTI-FLAG® Clone SIGI-25

Anticorpo monoclonale ANTI-FLAG® M2 1.0-1.2 mg/mL, clone M2, affinity isolated antibody, buffered aqueous solution (50% glycerol, 10 mM sodium phosphate, and 150 mM NaCl, pH 7.4)

Sigma-Aldrich

F1804

Anticorpo monoclonale ANTI-FLAG® M2

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

biological source

rabbit

biological source

rabbit

biological source

rabbit

biological source

mouse

clone

polyclonal

clone

polyclonal

clone

SIG1-25, monoclonal

clone

M2, monoclonal

species reactivity

all

species reactivity

all

species reactivity

-

species reactivity

all

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

ascites fluid

antibody form

affinity isolated antibody

form

buffered aqueous solution

form

buffered aqueous solution

form

-

form

buffered aqueous solution (50% glycerol, 10 mM sodium phosphate, and 150 mM NaCl, pH 7.4)

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Classe di stoccaggio

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificati d'analisi (COA)

Lot/Batch Number

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Domande

1–9 di 9 domande  

  1. Can this AB (F7425) used for mouse 30um thick section. mouse will be perfused with 4% PFA and than cryopreserved. thanks

    1 risposta

    1. This product has been recommended for use in immunofluorescence and indirect immunofluorescence at 5-10 μg/mL using 293T cells transfected with a plasmid encoding FLAG-JNK. It has not been tested for use in IHC applications. The end-user will need to optimize the assay. Please see the link below to review the product datasheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/207/353/f7425dat-ms.pdf

      In general, antibodies work well on 5-15 uM sections, for 30 uM sections, blocking /permeabilization steps and antibody dilution, the incubation time will need to be optimized by end user. Please see the links below to review an alternative anti-Flag antibody that may be more suitable for this application. Note that this product is a mouse monoclonal antibody:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/175/747/f1804bul-ms.pdf
      https://www.sigmaaldrich.com/product/sigma/f1804

      Utile?

  2. Is there a recommended isotype control for this antibody?

    1 risposta

    1. Any rabbit IgG such as product I5006 would be an appropriate isotype control for this antibody.

      Utile?

  3. Is there any references for the use of Product F7465, ANTI-FLAG® from rabbit, in immunohistochemistry or western blotting?

    1 risposta

    1. K. Schafer and T. Braun, Monoclonal Anti-FLAG Antibodies React with a New Isoform of Rat Mg++ Dependent Protein Phosphatase. Biochemical and Biophysical Research Communications, 207, 708.

      Utile?

  4. Is Product F7425, ANTI-FLAG® from rabbit, a monoclonal or polyclonal?

    1 risposta

    1. F7425 is a polyclonal antibody from rabbit.

      Utile?

  5. What is the Department of Transportation shipping information for this product?

    1 risposta

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Utile?

  6. What is the concentration of Product F7425, ANTI-FLAG® from rabbit?

    1 risposta

    1. The concentration is 0.9 mg protein per ml.

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  7. What are the positive controls that can be used with Product F7425, ANTI-FLAG® from rabbit?

    1 risposta

    1. The positive controls that can be used are as follows:Amino-terminal FLAG-BAP fusion protein, Product No. P7582Carboxy-terminal FLAG-BAP fusion protein, Product No. P7457Amino-terminal Met-FLAG-BAP fusion protein, Product No. P5975.

      Utile?

  8. How much Product F7425, ANTI-FLAG® from rabbit, should be used in western blotting and ELISA applications?

    1 risposta

    1. For a Western blot from a PAGE run (preferably) under denaturing conditions, a 1:400 dilution of this antibody should be used.  A 1-10 μg/ml concentration should be used when using thie antibody in an ELISA application.

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  9. How to get rid of background when using Product F7465, ANTI-FLAG® from rabbit,?

    1 risposta

    1. To reduce non-specific background on tissue staining, a small amount of Triton X-100 (Product No. X100 or T8787), can be added to the wash buffers and antibody incubation buffers. Try a Triton X-100 concentration of 0.1% to start. Tween 20 (Product No. P5927) can also be used.

      Utile?

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    Western blotting

    I HAVE USED THIS ANTIBODY ALOT DURING MY RESEARCH. AND IT HAS WORKED PERFECTLY.

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