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G1N10

GenElute™ Mammalian Genomic DNA Miniprep Kits

sufficient for 10 purifications

Synonym(s):

Mammalian Genomic DNA Miniprep, Gen Elute

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Size/SKUAvailabilityPrice
1 kit

Available to ship TODAYfromMILWAUKEE

$57.20

About This Item

NACRES:
NA.55
UNSPSC Code:
41105501

$57.20

Available to ship TODAYDetails

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usage

sufficient for 10 purifications

Quality Segment

storage temp.

15-25°C

General description

The GenElute Mammalian Genomic DNA Purification Kit provides a simple and convenient method to isolate pure, high molecular weight DNA from a variety of mammalian sources. These kits use a silica-based membrane, specially selected for genomic DNA purification, in a convenient spin column format.

The starting materials are lysed in a chaotropic salt-containing solution to ensure the thorough denaturation of macromolecules. The addition of ethanol causes DNA to bind when the lysate is spun through a silica membrane in a microcentrifuge tube. After washing to remove the contaminants, the DNA is eluted in 200 μL of a Tris-EDTA solution. The expected yields of genomic DNA will vary depending on the amount and type of starting material used. DNA purified with the kit has an A260/A280 ratio between 1.6 and 1.9 and can be up to 50 kb in length.

The purified genomic DNA is ready for immediate use in downstream applications such as restriction digest, PCR, southern blots, and sequencing reactions.

Application

The purified genomic DNA is ready for immediate use in downstream applications such as:
  • restriction endonuclease digestions
  • PCR
  • Southern blots
  • sequencing reactions
  • cloning
  • ligation

Biochem/physiol Actions

The starting materials are lysed in a chaotropic salt-containing solution to ensure the thorough denaturation of macromolecules. Addition of ethanol causes DNA to bind when the lysate is spun through a silica membrane in a microcentrifuge tube. After washing to remove the contaminants, the DNA is eluted in 200 μL of a Tris-EDTA solution. The expected yields of genomic DNA will vary depending on the amount and type of starting material used. DNA purified with the kit has an A260/A280 ratio between 1.6 and 1.9 and can be up to 50 kb in length.

Features and Benefits

  • Expected yield: 25 μg from 2 x 106 cultured cells; 30 μg from 25 mg of tissue
  • Elution volume: 200 - 400 μl
  • Time required: 20 min after lysis
  • A260/A280 ratio: 1.6 - 1.9
  • Mechanical homogenization required: No

Other Notes

For additional information, please see www.sigma-aldrich.com/genomicdna.

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

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description

sufficient for 10 purifications

description

sufficient for 350 purifications

description

sufficient for 70 purifications

description

sufficient for 70 purifications

usage

sufficient for 10 purifications

usage

sufficient for 350 purifications

usage

sufficient for 70 purifications

usage

sufficient for 70 purifications

storage temp.

15-25°C

storage temp.

15-25°C

storage temp.

15-25°C

storage temp.

15-25°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

G1N70

G1N10, G1N9602, G1N70, G1N9604

G1N350, G1N10, G1N9602, G1N9604

G2N10, G2N350

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Kit Components Also Available Separately

Product No.
Description
SDS & Pricing
  • R6148
    RNase A solution
    SDS
  • P2308
    Proteinase K from Tritirachium album, lyophilized powder, Molecular Biology, BioUltra, ≥30 units/mg protein
    SDS
  • C2112
    Column Preparation Solution
    SDS

Signal Word

Danger

Target Organs

Respiratory system

Flash Point (°F)

483.8 °F

Flash Point (°C)

251 °C

WGK

WGK 3

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - ED ENV 1 - Eye Dam. 1 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Storage Class

10 - Combustible liquids

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Questions

  1. Is the DNA extracted from this kit going to be a complete or fragmented DNA?

    1 answer

    1. This purified product will not be intact gDNA, but rather fragments with high molecular weight fragments up to 50kDa. In order to obtain minimally fragmented gDNA you will likely need a protocol that utilized phenol chloroform extraction such as the one linked below.

      https://www.protocols.io/view/phenol-chloroform-dna-extraction-optimized-for-hmw-b6ysrfwe.html

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