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G8132

β-Glucuronidase from limpets (Patella vulgata)

Type L-II, lyophilized powder, 1,000,000-3,000,000 units/g solid

Synonym(s):

β-D-Glucuronide glucuronosohydrolase

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100000 units
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$85.50
250000 units
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$170.00
500000 units
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$284.00
1000000 units
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$365.00
2000000 units
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$582.00
5 x 1 ea
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$1,300.00

About This Item

CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-606-8
MDL number:
EC Number:
Specific activity:
1,000,000-3,000,000 units/g solid
Biological source:
limpet (Patella vulgata)

$85.50


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biological source

limpet (Patella vulgata)

Quality Segment

type

Type L-II

form

lyophilized powder

specific activity

1,000,000-3,000,000 units/g solid

shipped in

wet ice

storage temp.

−20°C

Application

β-Glucuronidase Type L-II has been shown to be a superior enzyme for the hydrolysis of drug-glucuronides from urine.
New Technical Article Comparing Performance of Different Enzymes
Learn more about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine
The enzyme from patella vulgata is reported to be more effective in hydrolyzing opioid-glucuronides than the Helix pomatia, bovine liver and E. coli enzymes

Biochem/physiol Actions

β-glucuronidase (β-GIc) is an exoglycosidase that catalyzes the breakdown of complex carbohydrates. In humans it converts conjugated bilirubin into the unconjugated form, making bilirubin suitable for reabsorption. Morphine 3-β-D glucuronide was converted to morphine using β-glucuronidase obtained from Patella vulgate.

Analysis Note

Contains sulfatase activity which is inhibited by 0.1 M phosphate.

Other Notes

One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at pH 3.8 (30 min assay).

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This Item
SRE0093G0762G0876
description

Type L-II, lyophilized powder, 1,000,000-3,000,000 units/g solid

description

aqueous solution

description

Type H-3AF, aqueous solution, ≥60,000 units/mL

description

Type H-2, aqueous solution, ≥85,000 units/mL

specific activity

1,000,000-3,000,000 units/g solid

specific activity

-

specific activity

≥60,000 units/mL

specific activity

≥85,000 units/mL

biological source

limpet (Patella vulgata)

biological source

-

biological source

Helix pomatia

biological source

-

form

lyophilized powder

form

aqueous solution

form

aqueous solution

form

aqueous solution

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

shipped in

wet ice

shipped in

ambient

shipped in

wet ice

shipped in

wet ice

Quality Level

300

Quality Level

300

Quality Level

300

Quality Level

300


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Pictograms

Health hazard

Signal Word

Danger

Hazard Codes

Precautionary Statements

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, type N95 (US)



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