The epitope has not been mapped. There is no direct data available on the reactivity of this product with A,B1 & B2 chains. However, in standard blotting procedures (5% gel) under non-reducing conditions, this product stains 2 bands at approximately 200kD and a broad band (or 2 bands) at 400kD, in a heat-denatured murine laminin preparation . As stated on the data sheet (see link below) the 900kD laminin molecule comprises one A chain (440kD), one B1 chain (215kD) and one B2 (205kD) held together by sulfide bonds. This may indicate reactivity with A,B1 and B2 chains.
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/258/875/l8271pis-ms.pdf
L8271
Anti-Laminin antibody, Mouse monoclonal
clone LAM-89, purified from hybridoma cell culture
Synonym(s):
Anti-Laminin Antibody
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About This Item
Conjugate:
unconjugated
Clone:
LAM-89, monoclonal
Application:
ELISA (i), IHC (p), WB
Citations:
71
Pricing and availability is not currently available.
Product Name
Anti-Laminin antibody, Mouse monoclonal, clone LAM-89, purified from hybridoma cell culture
biological source
mouse
Quality Segment
conjugate
unconjugated
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
LAM-89, monoclonal
contains
15 mM sodium azide
species reactivity
feline, human, pig
should not react with
rabbit, lizard, sheep, carp, canine, chicken, rat, guinea pig, goat, frog, snake
technique(s)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1,000 using enzyme-treated human tissue sections, indirect ELISA: suitable, western blot: suitable
isotype
IgG1
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... LAMA1(284217), LAMA2(3908), LAMB1(3912), LAMB2(3913)
General description
Laminin, a 900kD (approx.) complex extracellular glycoprotein, is the most abundant structural and biologically active component present in the basement membranes. It is a cell substrate adhesion protein and thus has a crucial role in promoting neurite regeneration and modulating cellular functions like differentiation, cell shape and cell movement. Monoclonal Anti-laminin antibody can be used in immunohistochemistry to classify various disease processes involving basement membranes, to mark blood vessel walls of different species and to determine the origin and classification of human tumours. It can also be used in dot blot assays. Mouse anti- laminin antibody reacts specifically with purified human laminin but not with collagen IV, fibronectin, vitronectin or chondroitin sulfate types A, B, and C.
Monoclonal Anti-Laminin (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Laminin, the most abundant structural and biologically active component in basement membranes, is a complex extracellular glycoprotein with an approximate molecular weight of 900 kDa. Laminin is composed of one A chain (400 kDa) one B1 chain (215 kDa) and one B2 chain (205 kDa) all held together by disulfide bonds. Laminin is only found in significant quantities in basement membranes, the thin extracellular matrices that surround epithelial tissue, nerve, fat cells and smooth, striated and cardiac muscle.
Immunogen
human laminin.
Application
Monoclonal Anti-Laminin antibody:
- immunohistochemistry to stain matrix proteins
- double immunofluorescence staining
- indirect immunofluorescence
- immunogold labelling for electron microscopy
Monoclonal Anti-laminin antibody can be used in indirect immunostaining to determine the distribution of laminin. It may also be used for ELISA, Immunofluorescence and western blotting.
Biochem/physiol Actions
Laminin is a cell substrate adhesion protein and thus has a crucial role in promoting neurite regeneration and modulating cellular functions like differentiation, cell shape and cell movement because it appears to be an important cell substrate adhesion protein. It plays an important role in many aspects of the cell biology. Variations in the expression of this protein have been observed in embryogenesis, organogenesis, post traumatic healing and cancer.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
1 of 1
This Item | |||
|---|---|---|---|
| clone LAM-89, monoclonal | clone polyclonal | clone LAM-89, monoclonal | clone 2F7, monoclonal |
| biological source mouse | biological source rabbit | biological source mouse | biological source mouse |
| species reactivity feline, human, pig | species reactivity animal, human | species reactivity feline, human, porcine | species reactivity human |
| conjugate unconjugated | conjugate unconjugated | conjugate - | conjugate unconjugated |
| Gene Information human ... LAMA1(284217), LAMA2(3908), LAMB1(3912), LAMB2(3913) | Gene Information human ... LAMA1(284217) | Gene Information cat ... Lama1(101083299) | Gene Information human ... LAMA5(3911) |
| antibody form purified from hybridoma cell culture | antibody form affinity isolated antibody | antibody form purified from hybridoma cell culture | antibody form purified immunoglobulin |
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Storage Class
12 - Non Combustible Liquids
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