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L8271

Anti-Laminin antibody, Mouse monoclonal

clone LAM-89, purified from hybridoma cell culture

Synonym(s):

Anti-Laminin Antibody

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100 μL

Available to ship TODAYfromMILWAUKEE

$552.00
0.2 mL

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$712.00

About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
MDL number:
MFCD00162586
Conjugate:
unconjugated
Clone:
LAM-89, monoclonal
Application:
ELISA (i), IHC (p), WB
Citations:
71

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Product Name

Anti-Laminin antibody, Mouse monoclonal, clone LAM-89, purified from hybridoma cell culture

biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

LAM-89, monoclonal

contains

15 mM sodium azide

species reactivity

feline, human, pig

should not react with

rabbit, lizard, sheep, carp, canine, chicken, rat, guinea pig, goat, frog, snake

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1,000 using enzyme-treated human tissue sections, indirect ELISA: suitable, western blot: suitable

isotype

IgG1

UniProt accession no.

P07942, P25391

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... LAMA1(284217), LAMA2(3908), LAMB1(3912), LAMB2(3913)

General description

Laminin, a 900kD (approx.) complex extracellular glycoprotein, is the most abundant structural and biologically active component present in the basement membranes. It is a cell substrate adhesion protein and thus has a crucial role in promoting neurite regeneration and modulating cellular functions like differentiation, cell shape and cell movement. Monoclonal Anti-laminin antibody can be used in immunohistochemistry to classify various disease processes involving basement membranes, to mark blood vessel walls of different species and to determine the origin and classification of human tumours. It can also be used in dot blot assays. Mouse anti- laminin antibody reacts specifically with purified human laminin but not with collagen IV, fibronectin, vitronectin or chondroitin sulfate types A, B, and C.
Monoclonal Anti-Laminin (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Laminin, the most abundant structural and biologically active component in basement membranes, is a complex extracellular glycoprotein with an approximate molecular weight of 900 kDa. Laminin is composed of one A chain (400 kDa) one B1 chain (215 kDa) and one B2 chain (205 kDa) all held together by disulfide bonds. Laminin is only found in significant quantities in basement membranes, the thin extracellular matrices that surround epithelial tissue, nerve, fat cells and smooth, striated and cardiac muscle.

Immunogen

human laminin.

Application

Monoclonal Anti-Laminin antibody:


  • immunohistochemistry to stain matrix proteins
  • double immunofluorescence staining
  • indirect immunofluorescence
  • immunogold labelling for electron microscopy
Monoclonal Anti-laminin antibody can be used in indirect immunostaining to determine the distribution of laminin. It may also be used for ELISA, Immunofluorescence and western blotting.

Biochem/physiol Actions

Laminin is a cell substrate adhesion protein and thus has a crucial role in promoting neurite regeneration and modulating cellular functions like differentiation, cell shape and cell movement because it appears to be an important cell substrate adhesion protein. It plays an important role in many aspects of the cell biology. Variations in the expression of this protein have been observed in embryogenesis, organogenesis, post traumatic healing and cancer.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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SAB4200719L9393WH0003915M1
Anti-Laminin antibody, Mouse monoclonal clone LAM-89, purified from hybridoma cell culture

Sigma-Aldrich

L8271

Anti-Laminin antibody, Mouse monoclonal

Anti-Laminin antibody, Mouse monoclonal clone LAM-89, purified from hybridoma cell culture

Sigma-Aldrich

SAB4200719

Anti-Laminin antibody, Mouse monoclonal

Anti-Laminin antibody produced in rabbit 0.5 mg/mL, affinity isolated antibody, buffered aqueous solution

Sigma-Aldrich

L9393

Anti-Laminin antibody produced in rabbit

Monoclonal Anti-LAMC1 antibody produced in mouse clone 2E6-B4, purified immunoglobulin, buffered aqueous solution

Sigma-Aldrich

WH0003915M1

Monoclonal Anti-LAMC1 antibody produced in mouse

species reactivity

feline, human, pig

species reactivity

feline, human, porcine

species reactivity

animal, human

species reactivity

human

Gene Information

human ... LAMA1(284217), LAMA2(3908), LAMB1(3912), LAMB2(3913)

Gene Information

cat ... Lama1(101083299)
human ... LAMA1(284217)
pig ... Lama1(100625589)

Gene Information

human ... LAMA1(284217)
mouse ... Lama1(16772)

Gene Information

human ... LAMC1(3915)

antibody form

purified from hybridoma cell culture

antibody form

purified from hybridoma cell culture

antibody form

affinity isolated antibody

antibody form

purified immunoglobulin

clone

LAM-89, monoclonal

clone

LAM-89, monoclonal

clone

polyclonal

clone

2E6-B4, monoclonal

conjugate

unconjugated

conjugate

-

conjugate

unconjugated

conjugate

unconjugated

biological source

mouse

biological source

mouse

biological source

rabbit

biological source

mouse

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Storage Class

12 - Non Combustible Liquids

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Certificates of Analysis (COA)

Lot/Batch Number
0000454053
0000454053
0000519046
0000519049
0000519049

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A Tezcaner et al.
Journal of biomedical materials research. Part A, 86(1), 170-181 (2007-10-25)
In developed countries the aging population faces increasing risks of blinding retinal diseases, for which there are few effective treatments available. Photoreceptor transplantation represents one approach, but generally results have been disappointing. We hypothesize that micropatterned biodegradable poly(L-lactic acid-co-glycolic acid)/poly(hydroxybutyrate-co-hydroxyvaleric
Laminin-211 in skeletal muscle function
Holmberg J, et al.
Cell adhesion & migration, 7(1), 111-111 (2013)
Andrea Farini et al.
Development (Cambridge, England), 143(4), 658-669 (2016-02-18)
Duchenne muscular dystrophy (DMD) is a progressive neuromuscular disorder characterized by muscle wasting and premature death. The defective gene is dystrophin, a structural protein, absence of which causes membrane fragility and myofiber necrosis. Several lines of evidence showed that in
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Global Trade Item Number

SKUGTIN
L8271-100UL04061837883736
L8271-.2ML04061833965948

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