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Anti-c-Myc antibody, Mouse monoclonal

Name: Anti-c-Myc antibody, Mouse monoclonal
Brand: SIGMA
Price: 574 USD
Availability: InStock

clone 9E10, purified from hybridoma cell culture

Synonym(s):

Anti-c-Myc

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Size/SKU	Availability	Price
100 μL	Check Cart for Availability	$574.00

About This Item

NACRES:

NA.56

UNSPSC Code:

12352203

Conjugate:

unconjugated

Clone:

9E10, monoclonal

Application:

ARR, ICC, IHC (p), IP, WB

Citations:

1472

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Properties

biological source

mouse

Quality Segment

200

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

9E10, monoclonal

form

buffered aqueous solution

mol wt

62 kDa

species reactivity

human

concentration

~2 mg/mL

technique(s)

immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, immunoprecipitation (IP): suitable, microarray: suitable, western blot: 1:5000 using c-myc tagged fusion protein

UniProt accession no.

P01106

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... MYC(4609)

Description

General description

c-Myc (v-myc avian myelocytomatosis viral oncogene homolog) is a transcription factor, which is coded by MYC gene. It was first described as a homolog of an avian retroviral oncogene. It belongs to the family of transcription factors which are characterized by basic region helix-loop-helix/leucine zipper. Along with N-Myc and L-Myc, it forms the MYC proto-oncogene family. It forms a heterodimer with MAX, and this heterodimer binds to the E-boxes/CACGTG sequence motif on DNA. This gene is localized to human chromosome 8q24.

The mouse Anti-c-MYC monoclonal antibody recognizes an epitope located within a sequence (EQKLISEEDL) of the product of the human oncogene c-myc.

Immunogen

synthetic peptide of the human p62^c-Myc protein.

Application

Monoclonal Anti-c-Myc antibody produced in mouse is suitable for immunoprecipitation, immunoblotting, ELISA, immunofluorescence, microarray and electron microscopy. It has also been used in ChIP (Chromatin Immunoprecipitation).

Biochem/physiol Actions

v-myc avian myelocytomatosis viral oncogene homolog belongs to MYC family. When overexpressed these proteins can cause DNA double-stand breaks (DSBs) and genome instability. This transcription factor MYC helps in regulating long noncoding RNAs (lncRNAs) which has been implicated in cancer cell proliferation and tumorigenesis. Its overexpression is also associated with various cancers, and in particular B cell lymphomas. Increased expression of c-MYC has a negative effect on the ability of B cell lymphomas to functionally present Ags/peptides to CD4⁺ T cells. This protein exerts suppressive effects at several critical checkpoints in Ag presentation. It is associated with Burkitt lymphoma and a group of diffuse large B-cell lymphoma (DLBCL).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Preparation Note

Working dilution: 1:5000

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

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This Item	M5546	C6594	SAB4700447
Sigma-Aldrich M4439 Anti-c-Myc antibody, Mouse monoclonal	Sigma-Aldrich M5546 Monoclonal Anti-c-Myc antibody produced in mouse	Sigma-Aldrich C6594 Monoclonal Anti-c-Myc−Cy3^™ antibody produced in mouse	Sigma-Aldrich SAB4700447 Monoclonal Anti-MYC , (C-terminal) antibody produced in mouse
description clone 9E10, purified from hybridoma cell culture	description clone 9E10, ascites fluid	description clone 9E10, purified immunoglobulin, buffered aqueous solution	description clone 9E10, purified immunoglobulin, buffered aqueous solution
antibody form purified from hybridoma cell culture	antibody form ascites fluid	antibody form purified immunoglobulin	antibody form purified immunoglobulin
species reactivity human	species reactivity human	species reactivity human	species reactivity fusion proteins in all species, human
clone 9E10, monoclonal	clone 9E10, monoclonal	clone 9E10, monoclonal	clone 9E10, monoclonal
conjugate unconjugated	conjugate unconjugated	conjugate CY3 conjugate	conjugate unconjugated
biological source mouse	biological source mouse	biological source mouse	biological source mouse
technique(s) microarray: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, immunocytochemistry: suitable, western blot: 1:5000 using c-myc tagged fusion protein, immunoprecipitation (IP): suitable	technique(s) immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, indirect ELISA: suitable, western blot: 1:100 using c-myc tagged fusion protein, immunoprecipitation (IP): suitable	technique(s) direct immunofluorescence: 1:50 using formalin-fixed, paraffin-embedded human colon carcinoma tissue	technique(s) flow cytometry: suitable

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Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c