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M8403

McCoy’s 5A Medium

With sodium bicarbonate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

5A basal medium

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Size/SKUAvailabilityPrice
500 mL
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$44.80
6 x 500 mL
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$225.00
24 x 500 mL
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$810.00

About This Item

UNSPSC Code:
12352207
NACRES:
NA.75
EC Number:
200-655-4
MDL number:

$44.80


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Product Name

McCoy′s 5A Medium, Modified, with sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Quality Segment

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

L-glutamine: no
NaHCO3: 2.2 g/L
glucose: 3.0 g/L
phenol red: 0.011 g/L

shipped in

ambient

storage temp.

2-8°C

General description

McCoy′s 5A Medium is suitable for the primary culture of cells from a wide range of tissue. It is recommended for the production of viruses in primary cell cultures.

Application

McCoy′s 5A Medium has been used to maintain:

  • G-361 melanoma cells;
  • 87-MG cells
  • human colon carcinoma cell line HCT116

Biochem/physiol Actions

McCoy′s 5A Medium has been originally developed to support liver tumor cells by modification of the amino acids found in BME. This formulation has also been used to support the growth of primary cultures of bone marrow, skin, gingiva, kidney, omentum, adrenals, lung, spleen, rat embryos, and other cell types.

Preparation Note

Supplement with 0.22 g/L L-glutamine.

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M4892M9309CB_90010305
vibrant-m

McCoy

description

Modified, with sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

description

Modified, with L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

description

Modified, with L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

description

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 90010305, mouse unknown/unspecified, Fibroblast

form

liquid

form

powder

form

liquid

form

-

sterility

sterile-filtered

sterility

-

sterility

sterile-filtered

sterility

-

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

components

L-glutamine: no
NaHCO3: 2.2 g/L
glucose: 3.0 g/L
phenol red: 0.011 g/L

components

phenol red: 0.011 g/L
L-glutamine: 0.21915 g/L
glucose: 3.0 g/L (Dextro)
NaHCO3: no

components

NaHCO3: 2.2 g/L
L-glutamine: 0.21 g/L
phenol red: 0.011 g/L
glucose: 3.0 g/L (Dextro)

components

-

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

dry ice

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−196°C


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Storage Class

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable



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