M4892

McCoy′s 5A Medium

Modified, with L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

• Synonym(s): 5A basal medium
• MDL number: MFCD00217560
• UNSPSC Code: 12352207
• NACRES: NA.71

Properties

• Quality Level: 500
• form: powder
• technique(s): cell culture | mammalian: suitable
• components: phenol red: 0.011 g/L; L-glutamine: 0.21915 g/L; glucose: 3.0 g/L (Dextro); NaHCO₃: no
• shipped in: ambient
• storage temp.: 2-8°C

Description

General description

McCoy′s 5A Medium is recommended to produce viruses in primary cell cultures.

Application

McCoy′s 5A Medium has been used for culturing:

• human colon carcinoma cell lines (HCT116, RKO, FET, CBS, HCT116b, and TENN) to study the function of leucine-rich repeat-containing G-protein-coupled receptor (LGR5) in colon cancer metastasis
• human colorectal HCT116 cells to study miR-224 over-expression in cancer
• osteoblasts to study ion reactivity of calcium-deficient hydroxyapatite in standard cell culture media

Biochem/physiol Actions

McCoy′s 5A Medium has been originally developed to support liver tumor cells by modification of the amino acids found in BME. This formulation has also been used to support the growth of primary cultures of bone marrow, skin, gingiva, kidney, omentum, adrenals, lung, spleen, rat embryos, and other cell types.

Quantity

Formulated to contain 11.9 grams of powder per liter of medium.

Reconstitution

Supplement with 2.2 g/L sodium bicarbonate.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3