T6400
Tris-Borate-EDTA buffer
5× Concentrate
Synonym(s):
TBE buffer
Select a Size
| Size/SKU | Availability | Price |
|---|---|---|
4 L | Available to ship TODAYfromMILWAUKEE | $149.00 $111.75 |
About This Item
$111.75
List Price$149.00Save 25%Available to ship TODAYDetails
Quality Segment
sterility
0.2 μm filtered
form
solution
impurities
DNase and RNase, none detected
pH
8.2-8.4 (25 °C)
SMILES string
OB(O)O.NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O
InChI
1S/C10H16N2O8.C4H11NO3.BH3O3/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8;2-1(3)4/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2;2-4H
InChI key
OSBLTNPMIGYQGY-UHFFFAOYSA-N
Application
Dilution of the TBE stock concentrates to a 1× TBE running buffer results in a buffer containing 89 mM Tris-borate and 2 mM EDTA, pH 8.3. The 5× or 10× stocks may also be added to an acrylamide/bis-acrylamide stock solution for making the PAGE gel. Applied voltages of less than 5 V/cm (distance between the electrodes of the unit) are recommended for maximum resolution.
Packaging
Preparation Note
Other Notes
1 of 1
This Item | |||
|---|---|---|---|
| description 5× Concentrate | description 10× concentrate, BioReagent, Molecular Biology, non-sterile; 0.2 μm filtered | description DNase and RNase, none detected, BioReagent, suitable for electrophoresis, 10× concentrate | description pH 8.3, pHast Pack™, powder |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level - |
| form solution | form solution | form powder blend | form powder |
| pH 8.2-8.4 (25 °C) | pH - | pH 8.1-8.5 | pH 8.3 |
| impurities DNase and RNase, none detected | impurities - | impurities DNase and RNase, none detected | impurities - |
| sterility 0.2 μm filtered | sterility non-sterile; 0.2 μm filtered | sterility - | sterility - |
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