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Anti-Parvalbumin Antibody, clone 4G3 , ZooMAb^® Rabbit Monoclonal

Name: Anti-Parvalbumin Antibody, clone 4G3 , ZooMAb<SUP>®</SUP> Rabbit Monoclonal
Brand: SIGMA
Price: 245 USD
Availability: InStock

recombinant, expressed in HEK 293 cells

Sustainability features

Enhanced Validation

Synonym(s):

PV, Parvalbumin alpha

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Size/SKU	Availability	Price
25 μL	Available to ship TODAYfromMILWAUKEE	$245.00
4 x 25 μL	Available to ship TODAYfromMILWAUKEE	$506.00

About This Item

NACRES:

NA.41

UNSPSC Code:

12352203

Conjugate:

unconjugated

Clone:

4G3, recombinant monoclonal

Application:

ICC, IF, IHC, WB

Citations:

$245.00

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Properties

biological source

rabbit (recombinant)

Quality Segment

200

recombinant

expressed in HEK 293 cells

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

4G3, recombinant monoclonal

product line

ZooMAb^® learn more

form

lyophilized

mol wt

calculated mol wt 12.06 kDa, observed mol wt ~12 kDa

species reactivity

human, mouse, rat

packaging

antibody small pack of 25 μL

greener alternative product characteristics

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

sustainability

Sustainability features

technique(s)

immunocytochemistry: suitable, immunofluorescence: suitable, immunohistochemistry: suitable, western blot: suitable

isotype

IgG

UniProt accession no.

P20472

greener alternative category

shipped in

ambient

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

human ... PVALB(5816)
mouse ... Pvalb(19293)
rat ... Pvalb(25269)

Description

General description

Parvalbumin alpha (UniProt: P20472; also known as PV) is encoded by the PVALB gene (Gene ID: 5816) in human. Parvalbumin is a low molecular weight soluble protein that binds two calcium ions with high affinity and is implicated in intracellular calcium regulation and trafficking. It is composed almost entirely of two EF-hand domains (aa. 39-74 & 78-110) and is structurally related to calmodulin and troponin C. It serves as a slow Ca2+ buffer that affects the amplitude and time course of intracellular Ca2+ transients in terminals after an action potential, and hence may regulate short-term synaptic plasticity. During nervous system development, its expression is shown to accompany the maturation of functional circuits. Parvalbumin containing neurons are abundant in the cortex, the hippocampus, and the thalamus. It is present in GABAergic interneurons in the nervous system, especially the reticular thalamus. Parvalbumin interneurons are a subset of inhibitory GABAergic neurons that control the excitability of post-synaptic pyramidal neurons. Dysfunction or loss of parvalbumin interneurons has been implicated in cognitive deficits associated with several well-known psychiatric disorders, including schizophrenia and depression. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

This product aligns with one or more of the 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

Immunogen

GST/His-tagged full-length recombinant human Parvalbumin.

Application

Anti-Parvalbumin, clone 4G3 ZooMAb, Cat. No. ZRB1218, is a highly specific rabbit recombinant monoclonal antibody that targets Parvalbumin and has been tested for use in Immunocytochemistry, Immunofluorescence, Immunohistochemistry, and Western Blotting.

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Parvalbumin in rat and human brain tissue lysate.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Parvalbumin in human cerebellum and mouse cerebellum tissue sections.

Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected Parvalbumin in mouse cerebellum tissue sections.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Parvalbumin in PC12 cells.

Note: Actual optimal working dilutions must be determined by end user as specimens and experimental conditions may vary.

Biochem/physiol Actions

Clone 4G3 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Parvalbumin.

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Preparation Note

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	P3088	MABN1191	SAB4200545
Sigma-Aldrich ZRB1218 Anti-Parvalbumin Antibody, clone 4G3 , ZooMAb^® Rabbit Monoclonal	Sigma-Aldrich P3088 Monoclonal Anti-Parvalbumin antibody produced in mouse	Sigma-Aldrich MABN1191 Anti-Parvalbumin Antibody, clone 4E9.2	Sigma-Aldrich SAB4200545 Anti-Parvalbumin antibody, Mouse monoclonal
description recombinant, expressed in HEK 293 cells	description clone PARV-19, ascites fluid	description clone 4E9.2, from mouse	description clone PARV-19, purified from hybridoma cell culture
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
antibody form purified antibody	antibody form ascites fluid	antibody form purified antibody	antibody form purified from hybridoma cell culture
species reactivity human, mouse, rat	species reactivity fish, frog, rabbit, canine, feline, bovine, pig, human, goat, rat	species reactivity human, mouse, rat	species reactivity rat, human
biological source rabbit (recombinant)	biological source mouse	biological source mouse	biological source mouse
Gene Information human ... PVALB(5816) mouse ... Pvalb(19293) rat ... Pvalb(25269)	Gene Information human ... PVALB(5816) rat ... Pvalb(25269)	Gene Information human ... PVALB(5816) mouse ... Pvalb(19293) rat ... Pvalb(25269)	Gene Information human ... PVALB(5816) rat ... PVALB(25269)
clone 4G3, recombinant monoclonal	clone PARV-19, monoclonal	clone 4E9.2, monoclonal	clone PARV-19, monoclonal

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Storage Class

11 - Combustible Solids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)