Please consider that the condition of the stock enzyme is different from the reaction in which it is used. You will be using a small amount of enzyme in a buffered reaction that is adjusted to the optimal pH by the reaction buffer itself.
A0810
Endoglycosidase H from Streptomyces plicatus
recombinant, expressed in E. coli, buffered aqueous solution
Synonym(s):
β-N-Acetylglucosaminidase H
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| Size/SKU | Availability | Price |
|---|---|---|
1 unit | Check Cart for Availability | $715.00 |
About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.32
MDL number:
Recombinant:
expressed in E. coli
$715.00
Check Cart for Availability
recombinant
expressed in E. coli
Quality Segment
conjugate
(N-linked)
form
buffered aqueous solution
shipped in
wet ice
storage temp.
2-8°C
General description
Endoglycosidase H or endo-β-N-acetylglucosaminidase H is an glycohydrolase. It is produced by Streptomyces plicatus and other Streptomyces species.
Biochem/physiol Actions
Endoglycosidase H is involved in cleaving the N-linked glycans present between the two N-acetylglucosamine (GlcNAc) residues in the core of the glycan chain in high-mannose sugars.
Physical form
Solution in 20 mM Tris HCl, pH 7.5, containing 50 mM NaCl, 1 mM EDTA
Other Notes
One unit will release N-linked oligosaccharides from 1 μmole of denatured ribonuclease B per min at 37 °C at pH 5.5.
1 of 1
This Item | |||
|---|---|---|---|
| description recombinant, expressed in E. coli, buffered aqueous solution | description Endoglycosidase H, Streptomyces plicatus, Recombinant, E. coli cleaves between the two N-acetylglucosamine residues in the diacetylchitobiose core of the oligosaccharide. | description recombinant, expressed in E. coli, ≥16 U/mg, buffered aqueous solution | description recombinant, expressed in E. coli, 20 U/mg |
| form buffered aqueous solution | form liquid | form buffered aqueous solution | form solution |
| recombinant expressed in E. coli | recombinant expressed in E. coli | recombinant expressed in E. coli | recombinant expressed in E. coli |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| shipped in wet ice | shipped in wet ice | shipped in wet ice | shipped in wet ice |
| Quality Level 200 | Quality Level 100 | Quality Level 200 | Quality Level 200 |
| conjugate (N-linked) | conjugate (N-linked) | conjugate (N-linked) | conjugate (N-linked) |
Storage Class
12 - Non Combustible Liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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Could you provide guidance on adjusting the pH of a solution that has a pH of 7.5, even though the workable pH range mentioned in the data sheet is 5.0–6.0? Specifically, which acid would be best to use for adjusting the pH before using the solution?
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