This product is filled by density, with a target fill volume of 0.5 ml for the 25mg and 2.0 ml for the 100mg package sizes.
A1602
淀粉葡萄糖苷酶 来源于黑曲霉
ammonium sulfate suspension, ≥40 units/mg protein
同義詞:
1,4-α-
登入 檢視組織與合約價格。
選擇尺寸
變更視圖
| 給您/庫存單位 | 供貨能力 | 價格 |
|---|
關於此項目
Specific activity:
≥40 units/mg protein
暫時無法取得訂價和供貨情況
form
ammonium sulfate suspension
Quality Segment
specific activity
≥40 units/mg protein
shipped in
wet ice
storage temp.
2-8°C
General description
葡萄糖淀粉酶是一种二糖酶，属于α-葡萄糖苷酶的一员。它由几种曲霉菌产生。
Application
来自黑曲霉（Aspergillus niger ）的淀粉葡萄糖苷酶被用于水解 α-D-葡糖苷。它可用于酿造啤酒以及生产面包和果汁。Sigma的淀粉葡萄糖苷酶已被用于将糖原水解成葡萄糖单体，以研究骨骼肌中的脂质积累[1]。
源于黑曲霉 (Aspergillus niger) 的淀粉葡萄糖苷酶用于释放南极蠓幼虫匀浆中的葡萄糖。它在混合消化酶液中用于淀粉消化。
Biochem/physiol Actions
来自黑曲霉（Aspergillus niger）的淀粉葡萄糖苷酶能够水解低聚糖的 α-D-(1-4)、α-D-(1-6)和α-D-(1-3)糖苷键[2]。
Physical form
悬浮于3.2 M（NH4）2SO4溶液中，pH约为6.0
Other Notes
在pH 4.5、55 °C条件下，一单位酶在3 min内可从淀粉中释放出1.0 mg葡萄糖。
1 of 1
本產品 | |||
|---|---|---|---|
| description ammonium sulfate suspension, ≥40 units/mg protein | description lyophilized, powder, ~70 U/mg | description ≥260 U/mL, aqueous solution | description powder, white, ~120 U/mg |
| specific activity ≥40 units/mg protein | specific activity ~70 U/mg | specific activity ≥260 U/mL | specific activity ~120 U/mg |
| form ammonium sulfate suspension | form powder | form aqueous solution | form powder |
| shipped in wet ice | shipped in - | shipped in - | shipped in - |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 100 |
Still not finding the right product?
Explore all of our products under 淀粉葡萄糖苷酶 来源于黑曲霉
存儲類別/等級
12 - Non Combustible Liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
-
What volume of ammonium sulfate is the 25mg suspended in?
1 answer-
Helpful?
-
-
How can a 1mg/mL solution of amyloglucosidase A1602 be prepared in acetate buffer?
1 answer-
The product A1602 is in an ammonium sulfate solution. Here's how you can work with this product:
- If the ammonium sulfate might interfere with the reaction(s):
1. Remove the enzyme from the refrigerator, ensuring that the ammonium sulfate suspensions are not frozen.
2. Gently invert the vial or bottle to produce a milky suspension. Avoid using harsh treatments like vortexing or sonicating, as they may denature some of the enzyme.
3. Use a pipette and sterile wide-bore pipette tip to remove a portion of the suspension.
4. Transfer the portion to a clean microcentrifuge or centrifuge tube.
5. Pellet the enzyme by centrifuging at approximately 10,000 – 15,000 x g for 10 minutes, if possible, using a refrigerated microcentrifuge set to a temperature between 2 °C and 8 °C.
6. Carefully remove as much clear supernatant as possible, retaining it; it is not necessary to remove absolutely all of the ammonium sulfate solution.
7. Dissolve the pellet by adding an appropriate amount of reaction buffer.
8. Assay the supernatant and the solution from the pellet for protein content and/or enzyme activity.- If the ammonium sulfate will not interfere with the reaction(s), follow the same steps as above.
It's important to note that for an ammonium sulfate suspension, most of the enzyme will be in solid form, and only negligible amounts of enzyme will likely be in the ammonium sulfate solution.
Alternatively, there are several Amyloglucosidase enzymes available in solid form (e.g., Product A7420 and A9228) that can be suspended in a solvent of your choice.
Helpful?
-