Skip to Content
MilliporeSigma

Key Documents

View All Documentation

Skip To

A1902

Anti-Mouse IgG (whole molecule)−Alkaline Phosphatase antibody produced in rabbit

affinity isolated antibody, buffered aqueous glycerol solution

Synonym(s):

Rabbit Anti-Mouse IgG (whole molecule)−AP

Sign In to View Organizational & Contract Pricing.

Select a Size

Change View
Pack SizeSKUAvailabilityPrice
1 mL
Please contact Customer Service for Availability
$560.00

About This Item

UNSPSC Code:
12352203
NACRES:
NA.46
MDL number:
MFCD00162640
Conjugate:
alkaline phosphatase conjugate
Clone:
polyclonal
Application:
ELISA (d), WB
Citations:
25

$560.00

Please contact Customer Service for Availability
​
Request a Bulk Order
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

biological source

rabbit

Quality Level

200

conjugate

alkaline phosphatase conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous glycerol solution

species reactivity

mouse

technique(s)

direct ELISA: 1:1,000, western blot: suitable

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

General description

Mouse IgG is a plasma B cell derived antibody isotype defined by its heavy chain. IgG is the most abundant antibody isotype found in mouse serum.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Enzyme-linked immunosorbent assay (1 paper)
Western Blotting (1 paper)
Rabbit anti-Mouse IgG (whole molecule)-alkaline phosphatase antibody has been used for ELISA and western blot assays.
Western blot analysis of lysates from the placental cell line 3ASubE-P-3 and U037 cells were performed using alkaline phosphatase conjugated rabbit anti- mouse IgG as the secondary at 1:2000 to recognize the mouse mom Il-8RB. Secondary was developed using bromochloroindoyl phosphate (Sigma) pH. 9.5 for 10-15 minutes.Western blot analysis of coimmunoprecipitates from human cell or rat cell vaginal extracts were performed using alkaline phosphatase conjugated rabbit anti- mouse IgG as the secondary at 1:15000 dilution.

Biochem/physiol Actions

IgG has an ability to cross the placental barrier. It acts as a complement activator and binds to the Fc-receptors on phagocytic cells. The level of IgG may vary with the status of disease or infection.
Rabbit polyclonal anti-Mouse IgG (whole molecule)-Alkaline Phosphatase reacts with mouse IgA, IgG1, IgG2a, IgG2b, IgG3, and IgM myeloma proteins.

Physical form

Solution in 0.05 M Tris, pH 8.0, and 1 mM MgCl2 containing 1% bovine serum albumin, 50% glycerol, and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Compare Similar Items

View Full Comparison

Show Differences

1 of 1

This Item
A4312A3688A3812
Anti-Mouse IgG (whole molecule)−Alkaline Phosphatase antibody produced in rabbit affinity isolated antibody, buffered aqueous glycerol solution

Sigma-Aldrich

A1902

Anti-Mouse IgG (whole molecule)−Alkaline Phosphatase antibody produced in rabbit

Anti-Mouse IgG (whole molecule)−Alkaline Phosphatase antibody produced in rabbit affinity isolated antibody, buffered aqueous glycerol solution

Sigma-Aldrich

A4312

Anti-Mouse IgG (whole molecule)−Alkaline Phosphatase antibody produced in rabbit

Anti-Mouse IgG (whole molecule)−Alkaline Phosphatase antibody produced in goat affinity isolated antibody, buffered aqueous glycerol solution

Sigma-Aldrich

A3688

Anti-Mouse IgG (whole molecule)−Alkaline Phosphatase antibody produced in goat

Anti-Rabbit IgG (whole molecule)–Alkaline Phosphatase antibody produced in goat affinity isolated antibody, buffered aqueous glycerol solution

Sigma-Aldrich

A3812

Anti-Rabbit IgG (whole molecule)–Alkaline Phosphatase antibody produced in goat

conjugate

alkaline phosphatase conjugate

conjugate

alkaline phosphatase conjugate

conjugate

alkaline phosphatase conjugate

conjugate

alkaline phosphatase conjugate

species reactivity

mouse

species reactivity

mouse

species reactivity

mouse

species reactivity

rabbit

biological source

rabbit

biological source

rabbit

biological source

goat

biological source

goat

technique(s)

direct ELISA: 1:1,000, western blot: suitable

technique(s)

direct ELISA: 1:30,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50, western blot: 1:30,000 using using β-Actin in total cell extract of HeLa cells (5-10 μg/well)

technique(s)

direct ELISA: 1:30,000, dot blot: 1:30,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50, western blot: 1:30,000

technique(s)

direct ELISA: 1:30,000, dot blot: 1:30,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50, western blot: 1:30,000

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

Still not finding the right product?

Explore all of our products under Anti-Mouse IgG (whole molecule)−Alkaline Phosphatase antibody produced in rabbit

— or —

Try our Product Selector Tool to narrow your options

Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0000487220
0000487220
0000458192
0000458192
0000453478

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Related Content

Data Sheet - A1902

Instructions

A M Robinson et al.
Biochimica et biophysica acta, 1369(2), 278-286 (1998-04-29)
Immunoliposomes have been prepared using antibodies raised to an antigenic determinant on the cell surface of the oral bacterium Streptococcus oralis (S. oralis) in an investigation of their potential to reduce dental plaque. The N-succinimidyl-S-acetylthioacetate (SATA) derivative of the antibodies
Nadia Belmellat et al.
Frontiers in immunology, 8, 1533-1533 (2017-12-01)
Tumor necrosis factor-alpha (TNF-α) blockade is an effective treatment for rheumatoid arthritis (RA) and other inflammatory diseases, but in patients, it is associated with reduced resistance to the infectious agents Mycobacterium tuberculosis and Listeria monocytogenes, among others. Our goal was
Michael J Abrams et al.
Proceedings of the National Academy of Sciences of the United States of America, 112(26), E3365-E3373 (2015-06-17)
What happens when an animal is injured and loses important structures? Some animals simply heal the wound, whereas others are able to regenerate lost parts. In this study, we report a previously unidentified strategy of self-repair, where moon jellyfish respond
View All Related Papers

Global Trade Item Number

SKUGTIN
A1902-5X1ML04061835804856
A1902-1ML04061837513893
A1902-.25ML04061835804832
A1902-.5ML04061835804849

Questions

Reviews

No rating value

Active Filters