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A1902

Anti-Mouse IgG (whole molecule)−Alkaline Phosphatase antibody produced in rabbit

affinity isolated antibody, buffered aqueous glycerol solution

Synonym(s):

Rabbit Anti-Mouse IgG (whole molecule)−AP

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Size/SKUAvailabilityPrice
1 mL

Available to ship TODAYfromMILWAUKEE

$389.00

About This Item

UNSPSC Code:
12352203
NACRES:
NA.46
MDL number:
MFCD00162640
Conjugate:
alkaline phosphatase conjugate
Clone:
polyclonal
Application:
ELISA (d), WB
Citations:
25

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biological source

rabbit

Quality Segment

200

conjugate

alkaline phosphatase conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous glycerol solution

species reactivity

mouse

technique(s)

direct ELISA: 1:1,000, western blot: suitable

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

General description

Mouse IgG is a plasma B cell derived antibody isotype defined by its heavy chain. IgG is the most abundant antibody isotype found in mouse serum.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Enzyme-linked immunosorbent assay (1 paper)
Western Blotting (1 paper)
Rabbit anti-Mouse IgG (whole molecule)-alkaline phosphatase antibody has been used for ELISA and western blot assays.
Western blot analysis of lysates from the placental cell line 3ASubE-P-3 and U037 cells were performed using alkaline phosphatase conjugated rabbit anti- mouse IgG as the secondary at 1:2000 to recognize the mouse mom Il-8RB. Secondary was developed using bromochloroindoyl phosphate (Sigma) pH. 9.5 for 10-15 minutes.Western blot analysis of coimmunoprecipitates from human cell or rat cell vaginal extracts were performed using alkaline phosphatase conjugated rabbit anti- mouse IgG as the secondary at 1:15000 dilution.

Biochem/physiol Actions

IgG has an ability to cross the placental barrier. It acts as a complement activator and binds to the Fc-receptors on phagocytic cells. The level of IgG may vary with the status of disease or infection.
Rabbit polyclonal anti-Mouse IgG (whole molecule)-Alkaline Phosphatase reacts with mouse IgA, IgG1, IgG2a, IgG2b, IgG3, and IgM myeloma proteins.

Physical form

Solution in 0.05 M Tris, pH 8.0, and 1 mM MgCl2 containing 1% bovine serum albumin, 50% glycerol, and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Anti-Mouse IgG (γ-chain specific)−Alkaline Phosphatase antibody produced in goat

description

affinity isolated antibody, buffered aqueous glycerol solution

description

affinity isolated antibody, buffered aqueous glycerol solution

description

affinity isolated antibody, buffered aqueous solution

description

affinity isolated antibody, buffered aqueous glycerol solution

biological source

rabbit

biological source

goat

biological source

goat

biological source

goat

technique(s)

direct ELISA: 1:1,000, western blot: suitable

technique(s)

western blot: 1:30,000, direct ELISA: 1:30,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50

technique(s)

direct ELISA: 1:7,000-1:21,000, western blot: suitable

technique(s)

direct ELISA: 1:30,000, dot blot: 1:30,000, western blot: 1:30,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50

conjugate

alkaline phosphatase conjugate

conjugate

alkaline phosphatase conjugate

conjugate

alkaline phosphatase conjugate

conjugate

alkaline phosphatase conjugate

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

species reactivity

mouse

species reactivity

-

species reactivity

mouse

species reactivity

mouse

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

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Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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